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- W4246862912 abstract "Most histological staining techniques for arterial tissues have only been applied to paraffin-embedded specimens. Due to the increasing use of endovascular stents, it has become necessary to evaluate vascular tissues using polymethyl methacrylate (PMMA)-embedding procedures to prevent the removal of implanted devices from the tissue and to avoid the disruption of the surrounding tissue. Therefore, we have modified three stains to be employed with PMMA-embedded, stented arteries: Safranin O for proteoglycan, von Kossa for calcium salts, and Gomori's iron reaction for iron ions. An etching procedure prior to staining was employed using hydrochloric acid and acid alcohol to allow for proper uptake of the stains. The three staining procedures were modified typically by extending time allotments within the various stains as compared with paraffin-embedded tissues. The stained PMMA-embedded arterial specimens are compared with routine paraffin-embedded arterial specimens. Although the resulting stains of PMMA-embedded sections produce slightly different colorations of structures as compared with paraffin-embedded sections, they do allow for the delineation of the desired structures: proteoglycans, calcium salts, and iron ions. These staining modifications provide a valuable addition to the evaluation of endovascular stented arteries. (The J Histotechnol 25:79, 2002)Submitted January 23, 2002; accepted March 4, 2002" @default.
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- W4246862912 date "2002-06-01" @default.
- W4246862912 modified "2023-10-14" @default.
- W4246862912 title "Evaluation of Endovascular Stents: Three Staining Procedures for Vascular Tissue Using Polymethyl Methacrylate Embedding" @default.
- W4246862912 doi "https://doi.org/10.1179/014788802794792284" @default.
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