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- W4246880013 abstract "We have developed a simple cell-free system for studying the stability of different mRNAs in vitro. We demonstrate that the threefold greater stability in vivo of truncated c-myc mRNA (lacking exon 1) compared with that of full-length c-myc mRNA is maintained in our in vitro system. Chimeric mRNAs in which the first exon of c-myc was fused to immunoglobulin C alpha heavy chain or glyceraldehyde-3-phosphate dehydrogenase mRNAs were not rapidly degraded, demonstrating that c-myc exon 1 alone is not sufficient to tag mRNAs for rapid degradation. Competition experiments show that full-length c-myc mRNA is specifically recognized by a factor(s) responsible for its rapid degradation. This system will allow further characterization and purification of these factors." @default.
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- W4246880013 date "1988-07-01" @default.
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- W4246880013 title "Differential stability of c-myc mRNAS in a cell-free system" @default.
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- W4246880013 doi "https://doi.org/10.1128/mcb.8.7.2860-2868.1988" @default.
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