Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246991693> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4246991693 endingPage "1950" @default.
- W4246991693 startingPage "1946" @default.
- W4246991693 abstract "We present the first experimental results obtained using a cryogenically-cooled Nb–Al2O3–Nb superconductor–insulator–superconductor (SIS) tunnel junction detector operating at 1.3 K as an ion detector in a time-of-flight mass spectrometer. As opposed to microchannel-plate ion detectors (MCPs) commonly used in such systems, cryogenic detectors such as SIS detectors offer a near 100% detection efficiency for all ions including single, very massive, slow-moving macromolecules. We describe the operating principle of an SIS detector and its use as an ion detector in our matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometer and compare its response to an MCP detector operated in the same system. To our knowledge, this is the first direct comparison of these detector types in this application. A comparison of count rates and time-of-flight spectra obtained with both detectors for human serum albumin (molecular weight 66 000 Da) indicates a two to three orders of magnitude higher detection efficiency per unit area for the SIS detector at this mass. For higher molecular masses we expect an even higher relative efficiency for cryogenic detectors since MCPs show a rapid decline in detection efficiency as ion mass increases, which is not expected to be the case for cryogenic detectors. Our results imply that time-of-flight techniques could be extended beyond the current upper mass limit if cryogenic detectors are used. Initially, cryogenic detectors will be used for the analysis of large protein molecules. If non-fragmenting ionization techniques can be perfected, cryogenic detectors will also open the possibility of the rapid analysis of large DNA molecules and perhaps intact microorganisms." @default.
- W4246991693 created "2022-05-12" @default.
- W4246991693 creator A5006357863 @default.
- W4246991693 creator A5020557158 @default.
- W4246991693 creator A5030706236 @default.
- W4246991693 creator A5045517098 @default.
- W4246991693 creator A5078908625 @default.
- W4246991693 creator A5084141932 @default.
- W4246991693 creator A5088778776 @default.
- W4246991693 date "1996-12-01" @default.
- W4246991693 modified "2023-09-27" @default.
- W4246991693 title "High‐efficiency Detection of 66 000 Da Protein Molecules Using a Cryogenic Detector in a Matrix‐assisted Laser Desorption/Ionization Time‐of‐flight Mass Spectrometer" @default.
- W4246991693 doi "https://doi.org/10.1002/(sici)1097-0231(199612)10:15<1946::aid-rcm774>3.3.co;2-v" @default.
- W4246991693 hasPublicationYear "1996" @default.
- W4246991693 type Work @default.
- W4246991693 citedByCount "1" @default.
- W4246991693 crossrefType "journal-article" @default.
- W4246991693 hasAuthorship W4246991693A5006357863 @default.
- W4246991693 hasAuthorship W4246991693A5020557158 @default.
- W4246991693 hasAuthorship W4246991693A5030706236 @default.
- W4246991693 hasAuthorship W4246991693A5045517098 @default.
- W4246991693 hasAuthorship W4246991693A5078908625 @default.
- W4246991693 hasAuthorship W4246991693A5084141932 @default.
- W4246991693 hasAuthorship W4246991693A5088778776 @default.
- W4246991693 hasConcept C104287447 @default.
- W4246991693 hasConcept C113196181 @default.
- W4246991693 hasConcept C120665830 @default.
- W4246991693 hasConcept C121332964 @default.
- W4246991693 hasConcept C145148216 @default.
- W4246991693 hasConcept C162356407 @default.
- W4246991693 hasConcept C16291436 @default.
- W4246991693 hasConcept C178790620 @default.
- W4246991693 hasConcept C185592680 @default.
- W4246991693 hasConcept C198291218 @default.
- W4246991693 hasConcept C33390570 @default.
- W4246991693 hasConcept C43617362 @default.
- W4246991693 hasConcept C54101563 @default.
- W4246991693 hasConcept C62520636 @default.
- W4246991693 hasConcept C89106999 @default.
- W4246991693 hasConcept C94915269 @default.
- W4246991693 hasConcept C98960154 @default.
- W4246991693 hasConceptScore W4246991693C104287447 @default.
- W4246991693 hasConceptScore W4246991693C113196181 @default.
- W4246991693 hasConceptScore W4246991693C120665830 @default.
- W4246991693 hasConceptScore W4246991693C121332964 @default.
- W4246991693 hasConceptScore W4246991693C145148216 @default.
- W4246991693 hasConceptScore W4246991693C162356407 @default.
- W4246991693 hasConceptScore W4246991693C16291436 @default.
- W4246991693 hasConceptScore W4246991693C178790620 @default.
- W4246991693 hasConceptScore W4246991693C185592680 @default.
- W4246991693 hasConceptScore W4246991693C198291218 @default.
- W4246991693 hasConceptScore W4246991693C33390570 @default.
- W4246991693 hasConceptScore W4246991693C43617362 @default.
- W4246991693 hasConceptScore W4246991693C54101563 @default.
- W4246991693 hasConceptScore W4246991693C62520636 @default.
- W4246991693 hasConceptScore W4246991693C89106999 @default.
- W4246991693 hasConceptScore W4246991693C94915269 @default.
- W4246991693 hasConceptScore W4246991693C98960154 @default.
- W4246991693 hasIssue "15" @default.
- W4246991693 hasLocation W42469916931 @default.
- W4246991693 hasOpenAccess W4246991693 @default.
- W4246991693 hasPrimaryLocation W42469916931 @default.
- W4246991693 hasRelatedWork W1972970445 @default.
- W4246991693 hasRelatedWork W1989316415 @default.
- W4246991693 hasRelatedWork W2001415670 @default.
- W4246991693 hasRelatedWork W2041311207 @default.
- W4246991693 hasRelatedWork W2051779653 @default.
- W4246991693 hasRelatedWork W2052494781 @default.
- W4246991693 hasRelatedWork W2085067469 @default.
- W4246991693 hasRelatedWork W2312196113 @default.
- W4246991693 hasRelatedWork W3121370778 @default.
- W4246991693 hasRelatedWork W4220916081 @default.
- W4246991693 hasVolume "10" @default.
- W4246991693 isParatext "false" @default.
- W4246991693 isRetracted "false" @default.
- W4246991693 workType "article" @default.