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- W4247061671 abstract "Summary. The 1- to 2-month-old female cross-breed cat presented with alopecia, erythema and many crusts were present on the tail. Microscopic examination of crusts from the tail disclosed epithelial debris, exudate, mycelium, and arthrospores. Microsporum gypseum which is rarely isolated from cats as a causative agent of dermatophytsis was cultured from the crusts on a 1/10 Sabouraud glucose agar at 27°C for 1 week. The isolate of M. gypseum from the cat was examined by random amplification of polymorphic DNA (RAPD), chitin synthase 1 gene (CHS1) sequence and mating experiments. The RAPD band patterns of the clinical isolate of M. gypseum was identical to those of tester strains of Arthroderma gypseum. Nucleotide sequence analysis of the CHS1 gene fragments from the isolate and a tester strain of A. gypseum showed 100% similarity. The mating experiments on the clinical isolate of M. gypseum completely agreed with the results from RAPD and CHS1 gene sequence. The isolate from the cat was confirmed to be A. gypseum (–) mating type, which was consistent with the result of mycological examination by molecular analyses. Zusammenfassung. Eine ein bis zwei Monate alte Mischlingskatze wurde mit Alopezie, Erythem und vielen Krusten am Schwanz vorgestellt. In den Krusten wurden mikroskopisch neben Epithelzelldetritus Myzelien und Arthrosporen nachgewiesen. Aus den Krusten wurde Microsporum gypseum isoliert, das bei Katzen selten als Dermatophytose-Erreger beobachtet wird. Das M. gypseum-Isolat wurde mittels RAPD, mit Chitinsynthase-1-Gensequenzierung und mit Matingexperimenten charakterisiert. Die RAPD-Bandenmuster des klinischen Isolats waren identisch mit denen des Testerstammes Arthroderma gypseum. Die Nukleotidsequenzanalyse des CHS1-Genfragments zeigte bei beiden Stämmen 100% Übereinstimmung. Auch die Matingexperimente ergaben Übereinstimmung. Demnach erwies sich das Isolat von der Katze als A. gypseum (–) Matingtyp in Übereinstimmung mit den molekularanalytischen Ergebnissen." @default.
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- W4247061671 date "2001-10-01" @default.
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- W4247061671 title "Microsporum gypseum isolated from a feline case of dermatophytosis" @default.
- W4247061671 doi "https://doi.org/10.1046/j.1439-0507.2001.d01-74.x" @default.
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