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- W4247075300 abstract "Abstract Site‐selective protein modification is a key step in facilitating protein functionalization and manipulation. To accomplish this, genetically engineered proteins were previously required, but the procedure was laborious, complex, and technically challenging. Herein we report the development of aptamer‐based recognition‐then‐reaction to guide site‐selective protein/DNA conjugation in a single step with outstanding selectivity and efficiency. As models, several proteins, including human thrombin, PDGF‐BB, Avidin, and His‐tagged recombinant protein, were studied, and the results showed excellent selectivity under mild reaction conditions. Taking advantage of aptamers as recognition elements with extraordinary selectivity and affinity, this simple preparation method can tag a protein in a complex milieu. Thus, with the aptamer obtained from cell‐SELEX, real‐time modification of live‐cell membrane proteins can be achieved in one step without any pre‐treatment." @default.
- W4247075300 created "2022-05-12" @default.
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- W4247075300 date "2017-08-25" @default.
- W4247075300 modified "2023-10-11" @default.
- W4247075300 title "Recognition-then-Reaction Enables Site-Selective Bioconjugation to Proteins on Live-Cell Surfaces" @default.
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- W4247075300 doi "https://doi.org/10.1002/ange.201706285" @default.
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