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- W4247081037 abstract "This chapter discusses a method for the enzymatic determination of sucrose. The determination of sucrose by measurements of the optical rotation after inversion requires apparatus, which cannot be provided by every laboratory. In addition, the accuracy is low if the sample contains small amounts of sucrose and large amounts of optically active compounds. In comparison, the enzymatic determination of sucrose is simple to carry out on any type of sample, and less than 10 μg sucrose can be estimated. The enzymatic determination is based on the principle that sucrose is hydrolyzed by invertase to glucose and fructose. The two hexoses are phosphorylated by ATP to the corresponding hexose-6-phosphates in the reaction catalyzed by hexokinase (HK). Fructose-6-phosphate is isomerized to glucose-6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate is oxidized by triphosphopyridine nucleotide (TPN) and glucoses-phosphate dehydrogenase (G6P-DH) to 6-phosphogluconate. Therefore, for each mole of sucrose 2 moles of reduced triphosphopyridine nucleotide (TPNH) are formed. The increase of optical density at 366 or 340 mμ because of the formation of TPNH is a measure of the over-all reaction. The reactions proceed rapidly and quantitatively if the measurements are made at the pH optima: invertase reaction at pH 4.6; and hexose determination at pH 7.6." @default.
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- W4247081037 date "1965-01-01" @default.
- W4247081037 modified "2023-10-14" @default.
- W4247081037 title "Sucrose" @default.
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- W4247081037 doi "https://doi.org/10.1016/b978-0-12-395630-9.50019-0" @default.
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