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- W4247133722 abstract "Abstract Background Mycoplasma gallisepticum ( MG ) is a major poultry pathogen that can induce Chronic Respiratory Disease (CRD) in chickens, causing serious economic losses in poultry industry worldwide. Increasing evidence suggests that microRNAs (miRNAs) act as a vital role in resisting microbial pathogenesis and maintaining cellular mechanism. Our previous miRNAs sequencing data showed that gga-miR-223 expression level significantly decreased in MG-infected chicken lungs. The aim of this study was to reveal the role of gga-miR-223 in MG-induced CRD progression. ResultsWe found that gga-miR-223 was remarkably down regulated and forkhead box O3 (FOXO3) was up-regulated in both MG-infected chicken embryos lungs and the chicken embryonic fibroblast cell line (DF-1) by qPCR. FOXO3 was verified as the target gene of gga-miR-223 through bioinformatics analysis and dual-luciferase reporter assay. Further studies showed that overexpressed gga-miR-223 could promote cell proliferation, cell cycle, and inhibit cell apoptosis by notably promoting the expression of cell cycle marker genes cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase 6 (CDK6) and Cyclin D1 (CCND1) and inhibiting the expression of apoptosis markers Bcl-2-like 11(BIM), FAS ligand (FASLG) and TNF-related apoptosis-inducing ligand (TRAIL). As expected, FOXO3 knockdown group got similar results. Overexpression of gga-miR-223 observably promoted cell multiplication, cell cycle progression, and inhibited apoptosis of MG -infected DF-1 cells, while inhibited gga-miR-223 had the opposite effect. ConclusionsTaken together, upon MG-infection, downregulated gga-miR-223 could decrease proliferation, cycle progression, and increase apoptosis through directly targeting FOXO3 to exert an aggravating MG-infectious effect." @default.
- W4247133722 created "2022-05-12" @default.
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- W4247133722 date "2020-10-08" @default.
- W4247133722 modified "2023-09-27" @default.
- W4247133722 title "Down-Regulated gga-miR-223 Inhibits Proliferation and Induces Apoptosis of MG-infected DF-1 Cells by Targeting FOXO3" @default.
- W4247133722 doi "https://doi.org/10.21203/rs.3.rs-86724/v1" @default.
- W4247133722 hasPublicationYear "2020" @default.
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