FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4247170089> ?p ?o ?g. }

• W4247170089 endingPage "747" @default.
• W4247170089 startingPage "736" @default.
• W4247170089 abstract "Purpose. Previous studies have suggested that corneal fibrosis controlled by a TGFβ-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFβ in post-PRK corneal wound healing as measured by in vivo Con-focal Microscopy Through Focusing (CMTF).Methods. Twelve rabbits received a monocular, 6-mm diameter, 9.0 D PRK myopic correction. Six animals received 50 μg of anti-TGFβ blocking antibodies applied topically 3×/day for three days post-PRK, while six animals received vehicle alone. An additional six animals served as unoperated controls. At various times during a four-month-period, animals were evaluated using CMTF, which generates a quantitative image intensity depth profile of the cornea. The location and reflectivity of corneal structures were identified from CMTF-profiles and used to determine epithelial and stromal thickness and corneal light reflectivity as an objective estimate of corneal haze. To correlate in vivo and ex vivo morphology, an additional six rabbits were analyzed at differing temporal intervals post-PRK for the expression and cytoskeletal organization of contractile micro-filaments: f-actin (stress fibers) and α-smooth muscle actin (a molecular marker for myofibroblast transformation).Results. Anti-TGFβ treated corneas showed significantly less CMTF measured light reflectivity (ANOVA, p < 0.02) following PRK compared to vehicle treated corneas with a 34% decrease at two weeks (2513 ± 758 U compared to 3810 ± 1262 U) and a 61% reduction in reflectivity at four months (447 ± 208 U compared to 1154 ± 585 U). The reduction in early development of light reflecting structures and the more rapid decline appeared related to anti-TGFβ-mediated inhibition of keratocyte activation and proliferation, myofibroblast transformation, and stromal fibrosis. Between anti-TGFβ and vehicle treated corneas, no significant differences were detected in either photoablation depth (126 ± 9 μm versus 126 ± 7 μm) or regression of photoablative effect (postoperative stromal thickening at four months: 95 ± 16 μm versus 95 ± 10 μm). Histologic examination demonstrated that regression of photoablative effect in anti-TGFβ treated corneas was related entirely to regeneration by corneal growth underlying the photoablated stromal surface. In vehicle treated corneas, fibrosis or deposition of new fibrotic tissue above the photoablated stromal surface was observed but contributed only about 25% of the total postoperative stromal thickening. No epithelial hyperplasia was detected. In unoperated control animals, a physiologic stromal thickening of 5 ± 2 μm per month (p > 0.001) was observed.Conclusions. This study confirms our earlier observations that increased corneal light reflectivity following PRK is predominantly due to: (1) distortion of the photoablated stromal surface leading to prominent reflections; and (2) increased reflections from activated and transformed keratocytes. Anti-TGFβ reduced keratocyte activation and transformation and inhibited stromal fibrosis, leading to a reduction in early light reflectivity as well as to a more rapid decline. Of greatest interest is the unexpected finding that anti-TGFβ treatment inhibited stromal fibrosis without reducing or delaying post-PRK stromal re-thickening. Based on these findings we propose that corneal thickness may be tightly and dynamically regulated by an unknown, non-TGFβ mediated pathway. We propose that anti-TGFβ treatment may be useful in reducing post-PRK corneal haze development in patients by: (1) inhibiting the recruitment of highly reflective, activated keratocytes, (2) inhibiting myofibroblast transformation, and 3) reducing stromal fibrosis. Curr. Eye Res. 17:736–747, 1998." @default.
• W4247170089 created "2022-05-12" @default.
• W4247170089 creator A5037247209 @default.
• W4247170089 creator A5078259243 @default.
• W4247170089 creator A5086298152 @default.
• W4247170089 creator A5091685408 @default.
• W4247170089 date "1998-01-01" @default.
• W4247170089 modified "2023-09-26" @default.
• W4247170089 title "Neutralizing antibody to TGF_βmodulates stromal fibrosis but not regression of photoablative effect following PRK" @default.
• W4247170089 cites W127609201 @default.
• W4247170089 cites W1494021554 @default.
• W4247170089 cites W1594050090 @default.
• W4247170089 cites W1868023684 @default.
• W4247170089 cites W1931924547 @default.
• W4247170089 cites W1947793024 @default.
• W4247170089 cites W1968936578 @default.
• W4247170089 cites W1991637232 @default.
• W4247170089 cites W1993478731 @default.
• W4247170089 cites W2000939333 @default.
• W4247170089 cites W2012337004 @default.
• W4247170089 cites W2014242579 @default.
• W4247170089 cites W2014930528 @default.
• W4247170089 cites W2016056870 @default.
• W4247170089 cites W2017027752 @default.
• W4247170089 cites W2021862836 @default.
• W4247170089 cites W2023231681 @default.
• W4247170089 cites W2026669914 @default.
• W4247170089 cites W2048392529 @default.
• W4247170089 cites W2052283590 @default.
• W4247170089 cites W2058097135 @default.
• W4247170089 cites W2070742211 @default.
• W4247170089 cites W2071341485 @default.
• W4247170089 cites W2088593768 @default.
• W4247170089 cites W2090697514 @default.
• W4247170089 cites W2102912833 @default.
• W4247170089 cites W2123550509 @default.
• W4247170089 cites W2150308074 @default.
• W4247170089 cites W2151711929 @default.
• W4247170089 cites W2157300659 @default.
• W4247170089 cites W2158550915 @default.
• W4247170089 cites W2243965861 @default.
• W4247170089 cites W2419350820 @default.
• W4247170089 cites W4295989308 @default.
• W4247170089 doi "https://doi.org/10.1080/02713689808951251" @default.
• W4247170089 hasPublicationYear "1998" @default.
• W4247170089 type Work @default.
• W4247170089 citedByCount "9" @default.
• W4247170089 countsByYear W42471700892016 @default.
• W4247170089 countsByYear W42471700892021 @default.
• W4247170089 countsByYear W42471700892022 @default.
• W4247170089 countsByYear W42471700892023 @default.
• W4247170089 crossrefType "journal-article" @default.
• W4247170089 hasAuthorship W4247170089A5037247209 @default.
• W4247170089 hasAuthorship W4247170089A5078259243 @default.
• W4247170089 hasAuthorship W4247170089A5086298152 @default.
• W4247170089 hasAuthorship W4247170089A5091685408 @default.
• W4247170089 hasConcept C118487528 @default.
• W4247170089 hasConcept C142724271 @default.
• W4247170089 hasConcept C150903083 @default.
• W4247170089 hasConcept C16930146 @default.
• W4247170089 hasConcept C185592680 @default.
• W4247170089 hasConcept C204232928 @default.
• W4247170089 hasConcept C207001950 @default.
• W4247170089 hasConcept C207865475 @default.
• W4247170089 hasConcept C26291073 @default.
• W4247170089 hasConcept C2776882836 @default.
• W4247170089 hasConcept C2779257441 @default.
• W4247170089 hasConcept C2780559512 @default.
• W4247170089 hasConcept C2780603455 @default.
• W4247170089 hasConcept C52124034 @default.
• W4247170089 hasConcept C71924100 @default.
• W4247170089 hasConcept C86803240 @default.
• W4247170089 hasConceptScore W4247170089C118487528 @default.
• W4247170089 hasConceptScore W4247170089C142724271 @default.
• W4247170089 hasConceptScore W4247170089C150903083 @default.
• W4247170089 hasConceptScore W4247170089C16930146 @default.
• W4247170089 hasConceptScore W4247170089C185592680 @default.
• W4247170089 hasConceptScore W4247170089C204232928 @default.
• W4247170089 hasConceptScore W4247170089C207001950 @default.
• W4247170089 hasConceptScore W4247170089C207865475 @default.
• W4247170089 hasConceptScore W4247170089C26291073 @default.
• W4247170089 hasConceptScore W4247170089C2776882836 @default.
• W4247170089 hasConceptScore W4247170089C2779257441 @default.
• W4247170089 hasConceptScore W4247170089C2780559512 @default.
• W4247170089 hasConceptScore W4247170089C2780603455 @default.
• W4247170089 hasConceptScore W4247170089C52124034 @default.
• W4247170089 hasConceptScore W4247170089C71924100 @default.
• W4247170089 hasConceptScore W4247170089C86803240 @default.
• W4247170089 hasIssue "7" @default.
• W4247170089 hasLocation W42471700891 @default.
• W4247170089 hasOpenAccess W4247170089 @default.
• W4247170089 hasPrimaryLocation W42471700891 @default.
• W4247170089 hasRelatedWork W2012240482 @default.
• W4247170089 hasRelatedWork W2023094989 @default.
• W4247170089 hasRelatedWork W2059115749 @default.
• W4247170089 hasRelatedWork W2078354178 @default.
• W4247170089 hasRelatedWork W2090662748 @default.
• W4247170089 hasRelatedWork W2091428132 @default.

• next