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- W4247187844 abstract "Five slime-forming Lactococcus lactis ssp. cremoris strains (ARH53, ARH74, ARH84, ARH87 and B30) isolated from Finnish fermented milk viili have been shown to produce viscous exopolysaccharides (EPSs) in skim milk cultures. The EPSs were separated and purified by treatment with trichloroacetic acid, ethanol precipitation and dialysis. All EPSs were anionic and composed of galactose, glucose and rhamnose of similar ratios. The 1H NMR spectrum of the EPS of strain ARH 74 showed high similarity with that of strain SBT 0495 EPS, previously reported to consist of pentasaccharide repeating units with a phosphodiester structural element. In dilute aqueous solutions (up to 0.1 g dl−1 of EPS), the EPSs showed polyelectrolyte effects, and they differed in viscosity. At a higher concentration of EPS (1%, w/v), the EPS solution showed a shear thinning behaviour, and the viscosity was temperature, pH and salt dependent. Addition of 1% EPS (w/v) to skim milk resulted in a significant increase in viscosity, and a gel formed at 40°C." @default.
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- W4247187844 date "1998-09-01" @default.
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- W4247187844 title "Purification of polysaccharides" @default.
- W4247187844 doi "https://doi.org/10.1016/s0958-2118(00)87479-0" @default.
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