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- W4247332172 abstract "Glucosyl-l-sorbose, -maltose, and -sucrose were synthesized using kojibiose phosphorylase (KPase) from Thermoanaerobacter brockii ATCC35047 with β-d-glucose-1-phosphate (β-G1P) as a glucosyl donor. One disaccharide and two trisaccharides thus synthesized were isolated by Toyopearl HW-40S column chromatography. The results of KPase digestion, methylation analysis, and 13C-NMR studies indicated that these oligosaccharides were α-d-glucopyranosyl-(1→5)-α-l-sorbopyranose, α-d-glucopyranosyl-(1→2)-α-d-glucopyranosyl-(1→4)-d-glucopyranose (4-α-d-kojibiosyl-glucose), and α-d-glucopyranosyl-(1→2)-α-d-glucopyranosyl-(1→2)-β-d-fructofuranoside, which are all novel oligosaccharides. Glucosyl-l-sorbose was partially hydrolyzed to glucose and l-sorbose by α-glucosidases, while glucosyl-sucrose and glucosyl-maltose were not hydrolyzed by glucoamylase, α-glucosidases, or CGTase." @default.
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- W4247332172 date "2001-01-01" @default.
- W4247332172 modified "2023-10-05" @default.
- W4247332172 title "Enzymatic Synthesis of Novel Oligosaccharides from <sc><font size = -1>L</font></sc>-Sorbose, Maltose, and Sucrose Using Kojibiose Phosphorylase" @default.
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- W4247332172 doi "https://doi.org/10.1263/jbb.92.173" @default.
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