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- W4247489963 abstract "Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation." @default.
- W4247489963 created "2022-05-12" @default.
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- W4247489963 date "2021-09-25" @default.
- W4247489963 modified "2023-10-17" @default.
- W4247489963 title "A xylose-utilizing Saccharomyces cerevisiae strain serves as a better host for producing acetyl-CoA derived n-butanol" @default.
- W4247489963 doi "https://doi.org/10.22541/au.163252821.18680330/v1" @default.
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