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- W4247689091 abstract "Trypanosoma brucei undergoes many morphological and biochemical changes during transformation from the bloodstream trypomastigote to the insect procyclic trypomastigote form. We cloned and determined the complete nucleotide sequence of a developmentally regulated cDNA. The corresponding mRNA was abundant in in vitro-cultivated procyclics but absent in bloodstream forms. The trypanosome genome contains eight genes homologous to this cDNA, arranged as four unlinked pairs of tandem repeats. The longest open reading frame of the cDNA predicts a protein of 15 kilodaltons, the central portion of which consists of 29 tandem glutamate-proline dipeptides. The repetitive region is preceded by an amino-terminal signal sequence and followed by a hydrophobic domain that could serve as a membrane anchor; the mRNA was found on membrane-bound polyribosomes. These results suggest that the protein is membrane associated." @default.
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- W4247689091 date "1987-08-01" @default.
- W4247689091 modified "2023-10-04" @default.
- W4247689091 title "Developmental regulation of a novel repetitive protein of Trypanosoma brucei." @default.
- W4247689091 doi "https://doi.org/10.1128/mcb.7.8.2838" @default.
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