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- W4247746322 abstract "Low Grade Gliomas (LGGs), although they are rare in incidence, usually affect younger adults (20-40 years of age) with patient survival varying between 5-15 years. The in vitro culturing of LGG and subsequent mutational profiling would be an invaluable tool for: (1) understanding mechanisms underpinning progression; (2) identifying novel diagnostic, prognostic and predictive biomarkers; and (3) identifying new treatment strategies using drug screening. Our aim is to develop and characterize patient derived LGG in vitro models through phenotypic, genetic and epigenetic analyses. Fresh LGG specimens were collected from the Prince of Wales Private Hospital, Sydney. All tumor samples were dissociated and cultured in serum free media supplemented with EGF and FGF. The minimum time to expand the cells from one passage to the next was at least 3 weeks. Successful cell lines were harvested at different passages. Molecular characterization of the tissue and cell lines were conducted using Sanger sequencing, Western blot, immunocytochemistry, short tandem repeat analyses and methylation arrays. 15 IDH1 mutant LGG cell lines were developed from 34 freshly collected specimens. IDH1 mutation exclusively reverted back to wild type in all cell lines within several passages. Further molecular characterization of LGG associated genes (for example p53, ATRX, TERT) and epigenetic studies through methylation arrays of paired tumor tissue samples and cell lines enabled us to better determine the selective pressures associated with in vitro culturing of patient derived glioma cell lines. We have established and characterized LGG cell lines derived from patient tumor material and provided a unique resource to better understand LGG biology and improve targeted treatment strategies whilst also detailing the extent to which in vitro models of patient derived glioma cell lines mimic their tumor of origin." @default.
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- W4247746322 date "2016-11-01" @default.
- W4247746322 modified "2023-10-16" @default.
- W4247746322 title "TMOD-14. ESTABLISHMENT AND MOLECULAR CHARACTERIZATION OF PATIENT DERIVED CELL LINES FROM LOW GRADE GLIOMA" @default.
- W4247746322 doi "https://doi.org/10.1093/neuonc/now212.884" @default.
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