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• W4247747034 abstract "We have investigated 123I and 125I DNA aptamer analogs of anticoagulant DNA aptamers to thrombin exosite 1 and exosite 2 for thrombus imaging potential. Two severe problems are rapid clearance from circulating blood and blood nuclease. With aptamers (unlike antisense) the nucleotide analogs used in polymerase chain reaction-selection cycles also must be used in the radiotracer. We investigated 3′-biotin-streptavidin (SA) bioconjugates of the aptamers to alleviate these problems. Blood nuclease assays and biodistribution analysis were used in the mouse and rabbit. We found that 3′-biotin protected the aptamers significantly from blood nuclease in vitro, but it did not slow in vivo clearance. In contrast, the 3′-biotin-SA bioconjugates were resistant to blood nuclease in vitro and were also longer-lived (10–20 times) in vivo. Bioconjugate aptamers retained affinity for thrombin. Two solutions emerge: 1) In noncirculating blood (within a thrombus) 3′-biotin extends aptamer lifetime, whereas 2) in circulating blood (the transport medium), where more aggressive clearance is encountered, 3′-SA extends aptamer lifetime." @default.
• W4247747034 created "2022-05-12" @default.
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• W4247747034 date "2000-04-01" @default.
• W4247747034 modified "2023-09-26" @default.
• W4247747034 title "Extending the lifetime of anticoagulant oligodeoxynucleotide aptamers in blood" @default.
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• W4247747034 doi "https://doi.org/10.1016/s0969-8051(99)00103-1" @default.
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