Matches in SemOpenAlex for { <https://semopenalex.org/work/W4247873834> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W4247873834 endingPage "128" @default.
- W4247873834 startingPage "122" @default.
- W4247873834 abstract "The tumor-suppressor gene p53 may transactivate the transcription of genes that down-regulate cellular growth-related genes and may become oncogenic as a result of the production of mutant proteins or the loss of its protein expression. This study reports that alterations of the highly conserved consensus intervening sequences at the splice junctions may lead to the inactivation of the p53 gene. Analyses with the combined polymerase chain reaction and single-strand conformational polymorphism and direct DNA sequencing of DNAs amplified by means of asymmetric polymerase chain reaction demonstrated sequence alterations at the splice junctions of introns 5 and 7 in four human hepatocellular carcinomas, with a single base substitution at the splice junction in three and a 10-bp deletion starting from the dinucleotide AG of the acceptor site of intron 5 in the fourth. Restriction fragment length polymorphism analysis disclosed allele loss in all three informative cases. The p53 mRNA concentrations were remarkably reduced or undetectable in two hepatocellular carcinomas, whereas the two tumors (cases 2 and 3) that had single base changes at the acceptor site of intron 7 had both normal and abnormally sized p53 mRNAs. Immunocytochemistry failed to detect the wild-type and mutant p53 proteins in all four tumors. Western-blot analysis disclosed an abnormal, larger p53 protein of 55 kD in the tumor of case 3. These findings suggest that the inactivation of p53 gene caused by the genetic alterations at the splice junction may occur more often than perceived and plays an important role in human hepatocarcinogenesis because of the inactivation of the p53 gene by way of the loss of the protein or production of an abnormal protein. (Hepatology 1994; 19:122–128)." @default.
- W4247873834 created "2022-05-12" @default.
- W4247873834 creator A5028520840 @default.
- W4247873834 date "1994-01-01" @default.
- W4247873834 modified "2023-10-18" @default.
- W4247873834 title "Genetic alterations at the splice junction of p53 gene in human hepatocellular carcinoma" @default.
- W4247873834 doi "https://doi.org/10.1016/0270-9139(94)90062-0" @default.
- W4247873834 hasPublicationYear "1994" @default.
- W4247873834 type Work @default.
- W4247873834 citedByCount "1" @default.
- W4247873834 countsByYear W42478738342013 @default.
- W4247873834 crossrefType "journal-article" @default.
- W4247873834 hasAuthorship W4247873834A5028520840 @default.
- W4247873834 hasBestOaLocation W42478738341 @default.
- W4247873834 hasConcept C104317684 @default.
- W4247873834 hasConcept C143065580 @default.
- W4247873834 hasConcept C153911025 @default.
- W4247873834 hasConcept C2778264664 @default.
- W4247873834 hasConcept C2780989783 @default.
- W4247873834 hasConcept C502942594 @default.
- W4247873834 hasConcept C54355233 @default.
- W4247873834 hasConcept C54458228 @default.
- W4247873834 hasConcept C555283112 @default.
- W4247873834 hasConcept C67705224 @default.
- W4247873834 hasConcept C86803240 @default.
- W4247873834 hasConcept C94671646 @default.
- W4247873834 hasConceptScore W4247873834C104317684 @default.
- W4247873834 hasConceptScore W4247873834C143065580 @default.
- W4247873834 hasConceptScore W4247873834C153911025 @default.
- W4247873834 hasConceptScore W4247873834C2778264664 @default.
- W4247873834 hasConceptScore W4247873834C2780989783 @default.
- W4247873834 hasConceptScore W4247873834C502942594 @default.
- W4247873834 hasConceptScore W4247873834C54355233 @default.
- W4247873834 hasConceptScore W4247873834C54458228 @default.
- W4247873834 hasConceptScore W4247873834C555283112 @default.
- W4247873834 hasConceptScore W4247873834C67705224 @default.
- W4247873834 hasConceptScore W4247873834C86803240 @default.
- W4247873834 hasConceptScore W4247873834C94671646 @default.
- W4247873834 hasIssue "1" @default.
- W4247873834 hasLocation W42478738341 @default.
- W4247873834 hasOpenAccess W4247873834 @default.
- W4247873834 hasPrimaryLocation W42478738341 @default.
- W4247873834 hasRelatedWork W1773520159 @default.
- W4247873834 hasRelatedWork W1978020758 @default.
- W4247873834 hasRelatedWork W2013123757 @default.
- W4247873834 hasRelatedWork W2085975240 @default.
- W4247873834 hasRelatedWork W2098325466 @default.
- W4247873834 hasRelatedWork W2112108933 @default.
- W4247873834 hasRelatedWork W2385130702 @default.
- W4247873834 hasRelatedWork W2955490813 @default.
- W4247873834 hasRelatedWork W3025081396 @default.
- W4247873834 hasRelatedWork W3101084134 @default.
- W4247873834 hasVolume "19" @default.
- W4247873834 isParatext "false" @default.
- W4247873834 isRetracted "false" @default.
- W4247873834 workType "article" @default.