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- W4247887029 abstract "Ischemia/Reperfusion (I/R) injuries are involved in many myocardial pathologic processes. Cardiac fibroblasts (CF) respond to this stress by excessive proliferation and secretion of soluble factors, e.g. ATP, leading to purinergic receptors activation. We previously showed that P2Y11 receptor (P2Y11R) played an immunomodulatory role in human dendritic cells (DC). We aimed to investigate the role of human CF (hCF) P2Y11R stressed by simulated I/R (Hypoxia/Reoxygenation or H/R) on immune response modulation and cardiac protection. Adult ventricular hCF were subjected to H/R (5 h at 1% O2 and 24 h at 21% O2), and P2Y11R specific agonist (NF546) and/or antagonist (NF340) were added at the onset of reoxygenation. Resulting hCF secretome (hCFS) was analyzed by bioluminescence and Mutiplex assays. DCs maturation markers and cytokines secretion were analyzed by flow cytometry and ELISA after 48 h incubation with hCFS and LPS stimulation. Human cardiomyocytes (CM) were subjected to 5 h/1 h H/R and hCFS was added at the onset of reoxygenation. CM viability/mortality was assessed by MTT assay and intracellular ATP quantification. hCF P2Y11R stimulation with NF546 significantly decreased H/R-induced proliferation and pro-inflammatory factors secretion. hCFS alone increased CD83, CD25 and CD86 expression in DC, suggesting a switch of DC from immature to mature phenotype. Under LPS stimulation, DCs seemed to be pro-inflammatory (high IL-12/IL-10 ratio) but hCFS induced a switch to tolerogenic profile (significant decrease of this ratio). hCFS prevented H/R-induced CM death via Akt phosphorylation. P2Y11R inhibition in hCF induced a complete loss of hCFS-induced protection in CM. Our data demonstrate a paracrine link between CF, CM and DC after I/R suggesting a key role of CF in the cellular responses to reperfusion. Therefore, P2Y11R modulates myocardial I/R injuries by acting on CF secretome." @default.
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- W4247887029 date "2018-04-01" @default.
- W4247887029 modified "2023-09-26" @default.
- W4247887029 title "Stimulation of P2Y11 receptor modulates cardiac fibroblasts secretome towards immunomodulatory and protective roles after simulated Ischemia/Reperfusion injury" @default.
- W4247887029 doi "https://doi.org/10.1016/j.acvdsp.2018.02.018" @default.
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