Matches in SemOpenAlex for { <https://semopenalex.org/work/W4247904703> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4247904703 endingPage "105" @default.
- W4247904703 startingPage "95" @default.
- W4247904703 abstract "The specificities of 28 commercially available compounds reported to be relatively selective inhibitors of particular serine/threonine-specific protein kinases have been examined against a large panel of protein kinases. The compounds KT 5720, Rottlerin and quercetin were found to inhibit many protein kinases, sometimes much more potently than their presumed targets, and conclusions drawn from their use in cell-based experiments are likely to be erroneous. Ro 318220 and related bisindoylmaleimides, as well as H89, HA1077 and Y 27632, were more selective inhibitors, but still inhibited two or more protein kinases with similar potency. LY 294002 was found to inhibit casein kinase-2 with similar potency to phosphoinositide (phosphatidylinositol) 3-kinase. The compounds with the most impressive selectivity profiles were KN62, PD 98059, U0126, PD 184352, rapamycin, wortmannin, SB 203580 and SB 202190. U0126 and PD 184352, like PD 98059, were found to block the mitogen-activated protein kinase (MAPK) cascade in cell-based assays by preventing the activation of MAPK kinase (MKK1), and not by inhibiting MKK1 activity directly. Apart from rapamycin and PD 184352, even the most selective inhibitors affected at least one additional protein kinase. Our results demonstrate that the specificities of protein kinase inhibitors cannot be assessed simply by studying their effect on kinases that are closely related in primary structure. We propose guidelines for the use of protein kinase inhibitors in cell-based assays." @default.
- W4247904703 created "2022-05-12" @default.
- W4247904703 creator A5010890113 @default.
- W4247904703 creator A5033866306 @default.
- W4247904703 creator A5056050624 @default.
- W4247904703 creator A5057473438 @default.
- W4247904703 date "2000-09-26" @default.
- W4247904703 modified "2023-10-11" @default.
- W4247904703 title "Specificity and mechanism of action of some commonly used protein kinase inhibitors" @default.
- W4247904703 doi "https://doi.org/10.1042/bj3510095" @default.
- W4247904703 hasPublicationYear "2000" @default.
- W4247904703 type Work @default.
- W4247904703 citedByCount "2602" @default.
- W4247904703 countsByYear W42479047032012 @default.
- W4247904703 countsByYear W42479047032013 @default.
- W4247904703 countsByYear W42479047032014 @default.
- W4247904703 countsByYear W42479047032015 @default.
- W4247904703 countsByYear W42479047032016 @default.
- W4247904703 countsByYear W42479047032017 @default.
- W4247904703 countsByYear W42479047032018 @default.
- W4247904703 countsByYear W42479047032019 @default.
- W4247904703 countsByYear W42479047032020 @default.
- W4247904703 countsByYear W42479047032021 @default.
- W4247904703 countsByYear W42479047032022 @default.
- W4247904703 countsByYear W42479047032023 @default.
- W4247904703 crossrefType "journal-article" @default.
- W4247904703 hasAuthorship W4247904703A5010890113 @default.
- W4247904703 hasAuthorship W4247904703A5033866306 @default.
- W4247904703 hasAuthorship W4247904703A5056050624 @default.
- W4247904703 hasAuthorship W4247904703A5057473438 @default.
- W4247904703 hasConcept C132149769 @default.
- W4247904703 hasConcept C184235292 @default.
- W4247904703 hasConcept C185592680 @default.
- W4247904703 hasConcept C195794163 @default.
- W4247904703 hasConcept C2778308172 @default.
- W4247904703 hasConcept C2780610907 @default.
- W4247904703 hasConcept C55493867 @default.
- W4247904703 hasConcept C57074206 @default.
- W4247904703 hasConcept C82495950 @default.
- W4247904703 hasConcept C86803240 @default.
- W4247904703 hasConcept C90934575 @default.
- W4247904703 hasConcept C95444343 @default.
- W4247904703 hasConcept C97029542 @default.
- W4247904703 hasConcept C99405784 @default.
- W4247904703 hasConceptScore W4247904703C132149769 @default.
- W4247904703 hasConceptScore W4247904703C184235292 @default.
- W4247904703 hasConceptScore W4247904703C185592680 @default.
- W4247904703 hasConceptScore W4247904703C195794163 @default.
- W4247904703 hasConceptScore W4247904703C2778308172 @default.
- W4247904703 hasConceptScore W4247904703C2780610907 @default.
- W4247904703 hasConceptScore W4247904703C55493867 @default.
- W4247904703 hasConceptScore W4247904703C57074206 @default.
- W4247904703 hasConceptScore W4247904703C82495950 @default.
- W4247904703 hasConceptScore W4247904703C86803240 @default.
- W4247904703 hasConceptScore W4247904703C90934575 @default.
- W4247904703 hasConceptScore W4247904703C95444343 @default.
- W4247904703 hasConceptScore W4247904703C97029542 @default.
- W4247904703 hasConceptScore W4247904703C99405784 @default.
- W4247904703 hasIssue "1" @default.
- W4247904703 hasLocation W42479047031 @default.
- W4247904703 hasOpenAccess W4247904703 @default.
- W4247904703 hasPrimaryLocation W42479047031 @default.
- W4247904703 hasRelatedWork W1983481482 @default.
- W4247904703 hasRelatedWork W1997855401 @default.
- W4247904703 hasRelatedWork W1999628035 @default.
- W4247904703 hasRelatedWork W2033421786 @default.
- W4247904703 hasRelatedWork W2037654470 @default.
- W4247904703 hasRelatedWork W2061404590 @default.
- W4247904703 hasRelatedWork W2064943475 @default.
- W4247904703 hasRelatedWork W2075521983 @default.
- W4247904703 hasRelatedWork W2151196184 @default.
- W4247904703 hasRelatedWork W4255520261 @default.
- W4247904703 hasVolume "351" @default.
- W4247904703 isParatext "false" @default.
- W4247904703 isRetracted "false" @default.
- W4247904703 workType "article" @default.