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- W424799279 abstract "目的：探讨人子宫蜕膜树突状细胞的分离方法和诱导培养的最佳条件,为研究树突状细胞在母胎免疫耐受中的作用奠定基础。方法：人早孕（7～12周）子宫蜕膜组织经机械破碎,梯度离心获得蜕膜低密度细胞,经粒-巨噬细胞集落刺激因子（GM-CSF）和干细胞因子（SCF）诱导,所获得细胞用FITC标记的抗人DEC-205、HLA-DR、CD11c抗体进行鉴定。结果：从蜕膜组织可获取足够数量蜕膜树突状细胞,体外培养10～18d细胞生长较为活跃,第14天左右达到最高值,维持3～4d开始下降,20d内细胞数仍能维持在（1.0～2.3）×10^5个/mL。结论：梯度离心法能成功分离蜕膜树突状前体细胞,GM-CSF和SCF体外诱导可提供蜕膜树突状细胞原代培养较为满意的生长条件。" @default.
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- W424799279 date "2007-01-01" @default.
- W424799279 modified "2023-09-24" @default.
- W424799279 title "人子宫蜕膜树突状细胞DEC-205^＋HLA-DR^＋CD11c^＋的分离纯化与鉴定" @default.
- W424799279 hasPublicationYear "2007" @default.
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