FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248054883> ?p ?o ?g. }

• W4248054883 abstract "Microbes, with their diverse metabolic capabilities and great adaptability, occupy almost every conceivable ecological niche on Earth – thus, could they survive in the oligotrophic (i.e., nutrient-poor) deionized (DI) water that we use for our experiments? Observations of white cauliflower-like lumps and black specks in salt solutions after months of storage in plastic bottles prompted the inquisition concerning the origin and nature of the “contaminants.” Hypothesising that the “contaminants” may be microbes from DI water, a series of growth experiments was conducted to detect and profile the microbial diversity in fresh DI water - produced on a just-in-time basis by a filter-cum-ion-exchange system with tap water as feed. While microbes could also be present on the surfaces and headspace of the unsterilized polyethylene bottles, investigating whether microbes are present in freshly produced DI water provides a more stringent performance test of the production system. Inoculation of DI water on R2A agar followed by multi-day aerobic cultivation revealed the presence of a wide variety of microbes (total viable cell concentration of ~10 3 colony forming units (CFU) per mL) with differing pigmentations, growth rates as well as colony sizes and morphologies. Additionally, greater abundance and diversity of microbes was recovered at 30 o C relative to 25 and 37 o C; most probably due to adaptation of microbes to tropical ambient water temperatures of 25 to 30 o C. Comparative experiments with tap water as inoculum recovered a significantly smaller number and diversity of microbes; thereby, suggesting that monochloramine residual disinfectant in tap water was effective in inhibiting cell viability. In contrast, possible removal of monochloramine by adsorption onto ion-exchange resins – and thus, alleviation of a source of environmental stress - might explain the observed greater diversity and abundance of viable microbes in DI water. Collectively, this study confirmed the presence of microbes in fresh DI water – and suggested a possible source for the “contaminants” in prepared salt solutions. Propensity of microbes for forming biofilm on various surfaces suggested that intermittent flow in just-in-time DI water production provided opportunities for cell attachment and biofilm formation in the system during water stagnation, and subsequent dislodgement and resuspension of cells upon water flow. Thus, regular maintenance and cleaning of the production system should help reduce DI water’s microbial load. Additionally, simple and low-cost culture experiments on agar medium can provide a qualitative and semi-quantitative estimate of microbial diversity and viable cell concentration in DI water, respectively; which, along with regular monitoring of water resistivity or conductivity, comprise a trio of tests useful for detecting possible contamination or, deterioration of DI water’s chemical and microbiological quality." @default.
• W4248054883 created "2022-05-12" @default.
• W4248054883 creator A5004074519 @default.
• W4248054883 date "2013-12-30" @default.
• W4248054883 modified "2023-10-16" @default.
• W4248054883 title "Microbes in deionized water: Implications for maintenance of laboratory water production system" @default.
• W4248054883 doi "https://doi.org/10.7287/peerj.preprints.181v1" @default.
• W4248054883 hasPublicationYear "2013" @default.
• W4248054883 type Work @default.
• W4248054883 citedByCount "0" @default.
• W4248054883 crossrefType "posted-content" @default.
• W4248054883 hasAuthorship W4248054883A5004074519 @default.
• W4248054883 hasBestOaLocation W42480548831 @default.
• W4248054883 hasConcept C107872376 @default.
• W4248054883 hasConcept C112570922 @default.
• W4248054883 hasConcept C142796444 @default.
• W4248054883 hasConcept C17538187 @default.
• W4248054883 hasConcept C185592680 @default.
• W4248054883 hasConcept C18903297 @default.
• W4248054883 hasConcept C31903555 @default.
• W4248054883 hasConcept C39432304 @default.
• W4248054883 hasConcept C523546767 @default.
• W4248054883 hasConcept C54355233 @default.
• W4248054883 hasConcept C86803240 @default.
• W4248054883 hasConcept C87717796 @default.
• W4248054883 hasConcept C93642007 @default.
• W4248054883 hasConceptScore W4248054883C107872376 @default.
• W4248054883 hasConceptScore W4248054883C112570922 @default.
• W4248054883 hasConceptScore W4248054883C142796444 @default.
• W4248054883 hasConceptScore W4248054883C17538187 @default.
• W4248054883 hasConceptScore W4248054883C185592680 @default.
• W4248054883 hasConceptScore W4248054883C18903297 @default.
• W4248054883 hasConceptScore W4248054883C31903555 @default.
• W4248054883 hasConceptScore W4248054883C39432304 @default.
• W4248054883 hasConceptScore W4248054883C523546767 @default.
• W4248054883 hasConceptScore W4248054883C54355233 @default.
• W4248054883 hasConceptScore W4248054883C86803240 @default.
• W4248054883 hasConceptScore W4248054883C87717796 @default.
• W4248054883 hasConceptScore W4248054883C93642007 @default.
• W4248054883 hasLocation W42480548831 @default.
• W4248054883 hasOpenAccess W4248054883 @default.
• W4248054883 hasPrimaryLocation W42480548831 @default.
• W4248054883 hasRelatedWork W11627789 @default.
• W4248054883 hasRelatedWork W18278206 @default.
• W4248054883 hasRelatedWork W18680549 @default.
• W4248054883 hasRelatedWork W22697194 @default.
• W4248054883 hasRelatedWork W22868761 @default.
• W4248054883 hasRelatedWork W24185336 @default.
• W4248054883 hasRelatedWork W25091709 @default.
• W4248054883 hasRelatedWork W3999918 @default.
• W4248054883 hasRelatedWork W4663350 @default.
• W4248054883 hasRelatedWork W8956736 @default.
• W4248054883 isParatext "false" @default.
• W4248054883 isRetracted "false" @default.
• W4248054883 workType "article" @default.