Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248092480> ?p ?o ?g. }
Showing items 1 to 52 of
52
with 100 items per page.
- W4248092480 endingPage "1097" @default.
- W4248092480 startingPage "1090" @default.
- W4248092480 abstract "S-adenosylmethionine synthetase (SAMS) catalyzes the formation of S-adenosylmethionine (SAM) and is essential to normal cell function. There are two forms of SAMS, liver-specific and nonliver-specific (often referred to as “kidney”), which are products of two different genes. SAMS isoenzymes differ greatly in kinetic parameters and sensitivity to inhibition by methionine analogs. The current work studied changes in SAMS and their significance in liver cancer. Northern blot analysis showed that while normal liver expresses only liver-specific SAMS, both HepG2 and HuH-7 cells express only nonliver-specific SAMS. Absence of liver-specific SAMS messenger RNA (mRNA) was not because of gene deletion or rearrangement but complete lack of gene transcription. Reverse-transcription polymerase chain reaction (RT-PCR) with liver-and kidney-specific SAMS primers showed that liver-specific SAMS mRNA was absent with only kidney SAMS mRNA present in HepG2, HuH-7, Hep3B, and HuH-1 cells, and four consecutive hepatocellular carcinoma (HCC) specimens. Normal liver tissues from the same patients express both forms of SAMS mRNA. As a result of the change in SAMS expression, SAMS activity was higher in HepG2 and HuH-7 cells at physiologically relevant methionine concentrations but lower at high (mmol/L) methionine concentrations than rat hepatocytes. Treatment with ethionine and seleno-D,L-ethionine, two inhibitors known to have I50 values 50 to 60 times lower against SAMS purified from Novikoff hepatoma cells as compared with SAMS purified from normal rat liver, resulted in increased cell lysis in HepG2 and HuH-7 cells but not cultured rat hepatocytes. These agents did not affect cellular adenosine triphosphate (ATP) levels but inhibited SAMS activity in HepG2 and HuH-7 cells when added to their protein extracts. In summary, expression of SAMS is altered in human liver cancer. This occurrence may provide a potentially exploitable target for cancer chemotherapy." @default.
- W4248092480 created "2022-05-12" @default.
- W4248092480 creator A5063174892 @default.
- W4248092480 date "1996-11-01" @default.
- W4248092480 modified "2023-10-13" @default.
- W4248092480 title "Changes in S-adenosylmethionine synthetase in human liver cancer: Molecular characterization and significance" @default.
- W4248092480 doi "https://doi.org/10.1053/jhep.1996.v24.pm0008903381" @default.
- W4248092480 hasPublicationYear "1996" @default.
- W4248092480 type Work @default.
- W4248092480 citedByCount "4" @default.
- W4248092480 countsByYear W42480924802012 @default.
- W4248092480 crossrefType "journal-article" @default.
- W4248092480 hasAuthorship W4248092480A5063174892 @default.
- W4248092480 hasBestOaLocation W42480924801 @default.
- W4248092480 hasConcept C104317684 @default.
- W4248092480 hasConcept C105580179 @default.
- W4248092480 hasConcept C153911025 @default.
- W4248092480 hasConcept C185592680 @default.
- W4248092480 hasConcept C2780486903 @default.
- W4248092480 hasConcept C2780912031 @default.
- W4248092480 hasConcept C515207424 @default.
- W4248092480 hasConcept C55493867 @default.
- W4248092480 hasConcept C86803240 @default.
- W4248092480 hasConceptScore W4248092480C104317684 @default.
- W4248092480 hasConceptScore W4248092480C105580179 @default.
- W4248092480 hasConceptScore W4248092480C153911025 @default.
- W4248092480 hasConceptScore W4248092480C185592680 @default.
- W4248092480 hasConceptScore W4248092480C2780486903 @default.
- W4248092480 hasConceptScore W4248092480C2780912031 @default.
- W4248092480 hasConceptScore W4248092480C515207424 @default.
- W4248092480 hasConceptScore W4248092480C55493867 @default.
- W4248092480 hasConceptScore W4248092480C86803240 @default.
- W4248092480 hasIssue "5" @default.
- W4248092480 hasLocation W42480924801 @default.
- W4248092480 hasOpenAccess W4248092480 @default.
- W4248092480 hasPrimaryLocation W42480924801 @default.
- W4248092480 hasRelatedWork W1980750043 @default.
- W4248092480 hasRelatedWork W1992150494 @default.
- W4248092480 hasRelatedWork W2019175827 @default.
- W4248092480 hasRelatedWork W2023084765 @default.
- W4248092480 hasRelatedWork W2073311733 @default.
- W4248092480 hasRelatedWork W2087181573 @default.
- W4248092480 hasRelatedWork W2325068706 @default.
- W4248092480 hasRelatedWork W2400392968 @default.
- W4248092480 hasRelatedWork W2410359522 @default.
- W4248092480 hasRelatedWork W3719474 @default.
- W4248092480 hasVolume "24" @default.
- W4248092480 isParatext "false" @default.
- W4248092480 isRetracted "false" @default.
- W4248092480 workType "article" @default.