Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248171531> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W4248171531 endingPage "tw176" @default.
- W4248171531 startingPage "tw176" @default.
- W4248171531 abstract "Maeno-Hikichi et al. investigated protein kinase C (PKC) regulation of Ca2+ channels and discovered an adaptor protein that targeted PKCε to N-type Ca2+ channels to form a functional signaling complex. At least 10 isozymes of PKC, a family of cytosolic kinases that translocate to the membrane after activation by diacylglycerol, are found in neurons. Although individual PKC isozymes are believed to mediate unique functions, the mechanisms underlying their specificity of action remain unclear. Maeno-Hikichi et al. used yeast two-hybrid screening of rat brain cDNA libraries to identify proteins that interacted with N-type but not P/Q-type Ca2+ channels and cloned enigma homolog (ENH), a protein known to bind PKC in vitro. Immunostaining revealed ENH expression in various brain regions and demonstrated that ENH was located in both neuronal cell bodies and presynaptic nerve terminals. The authors combined immunoprecipitation with Western analysis to demonstrate a PKCε-ENH-N-type Ca2+ channel complex. When coexpressed with Ca2+ channels in Xenopus oocytes, ENH facilitated PKC modulation of current through N-type but not P/Q-type Ca2+ channels; expression of chimeric channels confirmed that this facilitation of Ca2+ current depended on the Ca2+ channel's ENH-binding region. Disruption of the macromolecular complex with a peptide that inhibited PKCε translocation inhibited PKC facilitation of N-type Ca2+ current in hippocampal neurons. Thus, ENH is able to target a specific PKC isoform to a specific substrate, permitting selective modulation of Ca2+ current through a particular class of channels.Y. Maeno-Hikichi, S. Chang, K. Matsumura, M. Lai, H. Lin, N. Nakagawa, S. Kuroda, J.-F. Zhang, A PKCε-ENH-channel complex specifically modulates N-type Ca2+ channels. Nat. Neurosci. 6, 468-475 (2003). [Online Journal]" @default.
- W4248171531 created "2022-05-12" @default.
- W4248171531 date "2003-05-06" @default.
- W4248171531 modified "2023-10-18" @default.
- W4248171531 title "Selective Targeting of a PKC Isozyme" @default.
- W4248171531 doi "https://doi.org/10.1126/scisignal.1812003tw176" @default.
- W4248171531 hasPublicationYear "2003" @default.
- W4248171531 type Work @default.
- W4248171531 citedByCount "0" @default.
- W4248171531 crossrefType "journal-article" @default.
- W4248171531 hasConcept C104317684 @default.
- W4248171531 hasConcept C115302286 @default.
- W4248171531 hasConcept C119795356 @default.
- W4248171531 hasConcept C153911025 @default.
- W4248171531 hasConcept C181199279 @default.
- W4248171531 hasConcept C185592680 @default.
- W4248171531 hasConcept C195794163 @default.
- W4248171531 hasConcept C2779535977 @default.
- W4248171531 hasConcept C36020004 @default.
- W4248171531 hasConcept C53345823 @default.
- W4248171531 hasConcept C55493867 @default.
- W4248171531 hasConcept C62478195 @default.
- W4248171531 hasConcept C86803240 @default.
- W4248171531 hasConcept C95444343 @default.
- W4248171531 hasConcept C98539663 @default.
- W4248171531 hasConceptScore W4248171531C104317684 @default.
- W4248171531 hasConceptScore W4248171531C115302286 @default.
- W4248171531 hasConceptScore W4248171531C119795356 @default.
- W4248171531 hasConceptScore W4248171531C153911025 @default.
- W4248171531 hasConceptScore W4248171531C181199279 @default.
- W4248171531 hasConceptScore W4248171531C185592680 @default.
- W4248171531 hasConceptScore W4248171531C195794163 @default.
- W4248171531 hasConceptScore W4248171531C2779535977 @default.
- W4248171531 hasConceptScore W4248171531C36020004 @default.
- W4248171531 hasConceptScore W4248171531C53345823 @default.
- W4248171531 hasConceptScore W4248171531C55493867 @default.
- W4248171531 hasConceptScore W4248171531C62478195 @default.
- W4248171531 hasConceptScore W4248171531C86803240 @default.
- W4248171531 hasConceptScore W4248171531C95444343 @default.
- W4248171531 hasConceptScore W4248171531C98539663 @default.
- W4248171531 hasIssue "181" @default.
- W4248171531 hasLocation W42481715311 @default.
- W4248171531 hasOpenAccess W4248171531 @default.
- W4248171531 hasPrimaryLocation W42481715311 @default.
- W4248171531 hasRelatedWork W1969274607 @default.
- W4248171531 hasRelatedWork W1977185700 @default.
- W4248171531 hasRelatedWork W2011567844 @default.
- W4248171531 hasRelatedWork W2027126024 @default.
- W4248171531 hasRelatedWork W2031543068 @default.
- W4248171531 hasRelatedWork W2040908636 @default.
- W4248171531 hasRelatedWork W205064343 @default.
- W4248171531 hasRelatedWork W2114070275 @default.
- W4248171531 hasRelatedWork W2164887648 @default.
- W4248171531 hasRelatedWork W2413187894 @default.
- W4248171531 hasVolume "2003" @default.
- W4248171531 isParatext "false" @default.
- W4248171531 isRetracted "false" @default.
- W4248171531 workType "article" @default.