Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248396500> ?p ?o ?g. }
- W4248396500 endingPage "906" @default.
- W4248396500 startingPage "900" @default.
- W4248396500 abstract "Several lines of evidence suggest that nicotinic acetylcholine receptors (nAChRs) are a target of general anesthetics. Alphaxalone (5α-pregnan-3α-ol-11, 20-dion) is a neurosteroid, which was used clinically for anesthesia, but its effects on the function of nAChRs have not been well investigated. We examined the effects of alphaxalone on nAChRs in cultured bovine adrenal chromaffin cells. We studied the effects of alphaxalone on nicotine-induced increases in the cytosolic Ca2+ concentration ([Ca2+]i) and on membrane currents using Ca2+-imaging and whole-cell patch-clamp techniques, respectively, in these cells. We also examined the effects of alphaxalone on γ-aminobutyric acid A receptors in the same cells and compared them with the effects on nAChRs. Alphaxalone (0.1–100 μM) inhibited nicotine-induced [Ca2+]i increases in a concentration-dependent manner. Alphaxalone inhibited high K+-induced [Ca2+]i increases, but the inhibition was observed only at 100 μM. In voltage-clamp experiments using negative holding potentials, alphaxalone (0.1–100 μM) itself induced inward currents, which were abolished by the γ-aminobutyric acid A receptor antagonist picrotoxin. Alphaxalone also inhibited nicotine-induced inward currents, and the inhibition was unaffected by picrotoxin. We conclude that alphaxalone, at anesthetic concentrations, inhibits nAChRs in adrenal chromaffin cells. Alphaxalone may affect the sympathetic and other nervous systems via inhibition of nAChRs." @default.
- W4248396500 created "2022-05-12" @default.
- W4248396500 creator A5011719864 @default.
- W4248396500 creator A5036330211 @default.
- W4248396500 creator A5046273011 @default.
- W4248396500 creator A5062146842 @default.
- W4248396500 creator A5066981475 @default.
- W4248396500 creator A5067085986 @default.
- W4248396500 creator A5073066826 @default.
- W4248396500 creator A5075344730 @default.
- W4248396500 creator A5079974765 @default.
- W4248396500 date "2002-10-01" @default.
- W4248396500 modified "2023-10-16" @default.
- W4248396500 title "A Neurosteroid Anesthetic, Alphaxalone, Inhibits Nicotinic Acetylcholine Receptors in Cultured Bovine Adrenal Chromaffin Cells" @default.
- W4248396500 cites W1755477571 @default.
- W4248396500 cites W1903420193 @default.
- W4248396500 cites W1966844562 @default.
- W4248396500 cites W1974249330 @default.
- W4248396500 cites W1982815900 @default.
- W4248396500 cites W1985169379 @default.
- W4248396500 cites W1999250281 @default.
- W4248396500 cites W2000357170 @default.
- W4248396500 cites W2004149919 @default.
- W4248396500 cites W2008614256 @default.
- W4248396500 cites W2008740998 @default.
- W4248396500 cites W2009302789 @default.
- W4248396500 cites W2009415563 @default.
- W4248396500 cites W2018846121 @default.
- W4248396500 cites W2022514816 @default.
- W4248396500 cites W2022976720 @default.
- W4248396500 cites W2023282570 @default.
- W4248396500 cites W2029708659 @default.
- W4248396500 cites W2030293146 @default.
- W4248396500 cites W2036833970 @default.
- W4248396500 cites W2068260771 @default.
- W4248396500 cites W2081025398 @default.
- W4248396500 cites W2099943892 @default.
- W4248396500 cites W2113217079 @default.
- W4248396500 cites W2148176139 @default.
- W4248396500 cites W2150177510 @default.
- W4248396500 cites W2162067039 @default.
- W4248396500 doi "https://doi.org/10.1213/00000539-200210000-00020" @default.
- W4248396500 hasPublicationYear "2002" @default.
- W4248396500 type Work @default.
- W4248396500 citedByCount "8" @default.
- W4248396500 countsByYear W42483965002015 @default.
- W4248396500 countsByYear W42483965002022 @default.
- W4248396500 crossrefType "journal-article" @default.
- W4248396500 hasAuthorship W4248396500A5011719864 @default.
- W4248396500 hasAuthorship W4248396500A5036330211 @default.
- W4248396500 hasAuthorship W4248396500A5046273011 @default.
- W4248396500 hasAuthorship W4248396500A5062146842 @default.
- W4248396500 hasAuthorship W4248396500A5066981475 @default.
- W4248396500 hasAuthorship W4248396500A5067085986 @default.
- W4248396500 hasAuthorship W4248396500A5073066826 @default.
- W4248396500 hasAuthorship W4248396500A5075344730 @default.
- W4248396500 hasAuthorship W4248396500A5079974765 @default.
- W4248396500 hasBestOaLocation W42483965001 @default.
- W4248396500 hasConcept C126322002 @default.
- W4248396500 hasConcept C134018914 @default.
- W4248396500 hasConcept C147944092 @default.
- W4248396500 hasConcept C168258287 @default.
- W4248396500 hasConcept C170493617 @default.
- W4248396500 hasConcept C188987157 @default.
- W4248396500 hasConcept C2775894120 @default.
- W4248396500 hasConcept C2776885963 @default.
- W4248396500 hasConcept C2777597313 @default.
- W4248396500 hasConcept C2779324764 @default.
- W4248396500 hasConcept C2779547902 @default.
- W4248396500 hasConcept C2780910648 @default.
- W4248396500 hasConcept C44796713 @default.
- W4248396500 hasConcept C71924100 @default.
- W4248396500 hasConcept C80161118 @default.
- W4248396500 hasConcept C98274493 @default.
- W4248396500 hasConceptScore W4248396500C126322002 @default.
- W4248396500 hasConceptScore W4248396500C134018914 @default.
- W4248396500 hasConceptScore W4248396500C147944092 @default.
- W4248396500 hasConceptScore W4248396500C168258287 @default.
- W4248396500 hasConceptScore W4248396500C170493617 @default.
- W4248396500 hasConceptScore W4248396500C188987157 @default.
- W4248396500 hasConceptScore W4248396500C2775894120 @default.
- W4248396500 hasConceptScore W4248396500C2776885963 @default.
- W4248396500 hasConceptScore W4248396500C2777597313 @default.
- W4248396500 hasConceptScore W4248396500C2779324764 @default.
- W4248396500 hasConceptScore W4248396500C2779547902 @default.
- W4248396500 hasConceptScore W4248396500C2780910648 @default.
- W4248396500 hasConceptScore W4248396500C44796713 @default.
- W4248396500 hasConceptScore W4248396500C71924100 @default.
- W4248396500 hasConceptScore W4248396500C80161118 @default.
- W4248396500 hasConceptScore W4248396500C98274493 @default.
- W4248396500 hasIssue "4" @default.
- W4248396500 hasLocation W42483965001 @default.
- W4248396500 hasLocation W42483965002 @default.
- W4248396500 hasOpenAccess W4248396500 @default.
- W4248396500 hasPrimaryLocation W42483965001 @default.
- W4248396500 hasRelatedWork W1978592274 @default.
- W4248396500 hasRelatedWork W2000110691 @default.
- W4248396500 hasRelatedWork W2055762844 @default.