FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248442972> ?p ?o ?g. }

• W4248442972 endingPage "R264" @default.
• W4248442972 startingPage "R263" @default.
• W4248442972 abstract "Who or what is Xenopus? Xenopus (literally ‘strange foot’) is a genus of aquatic frogs that are native to southern Africa. These frogs are remarkably tolerant to starvation, disease, and other insults, allowing the genus to span more than 45 degrees of latitude in Sub-Saharan Africa. This formidable constitution also makes them very easy to keep in captivity, and thus Xenopus frogs are very widely used as model organisms for biomedical research (Figure 1). Each Xenopus female lays thousands of eggs at one time, which has led to the development of cell-free systems for biochemistry, molecular and cell biology studies. Moreover, fertilizing a clutch of eggs produces huge numbers of synchronized embryos, making Xenopus a widely used model for developmental biology. Finally, the large size of Xenopus oocytes has made them an important tool for studies of ion channels and transporters. Most research is done on Xenopus laevis — the species often colloquially referred to simply as ‘Xenopus’. However, in recent years, its sister species Xenopus tropicalis has emerged as a genetic model organism, owing to its simpler genome, faster life cycle and smaller size. What got all this started? Amphibians have long been used in developmental biology, but Xenopus became the predominant amphibian model due to their worldwide distribution to medical centers for pregnancy testing (urine from pregnant women induces ovulation in Xenopus). Curiously, wild Xenopus were traditionally used in Africa as a source of fertility medicine. This is quaint, but why use Xenopus today? Xenopus has a host of advantages that complement those of other model systems. First, Xenopus is really cheap. Second, the embryos can be observed developing from the first cell division to a tadpole in about 36 hours. Finally, one can easily manipulate gene function in large numbers of embryos by using morpholino-modified antisense oligos and by mis-expression of mRNA. This is particularly handy for testing the function of gene variants such as those associated with human disease. Transgenesis is also routine, as are large-scale small molecule screens. Sounds a bit like a zebrafish then… It does, though it has many complementary advantages for developmental biology. Perhaps the more relevant point is that Xenopus is also widely used by cell and molecular biologists because it provides a unique cell-free system for biochemical studies. You mentioned cell-free systems, are you saying Xenopus is even useful when mashed up? Indeed, Xenopus egg extracts have allowed the study of many complicated cellular events in a test tube. Large quantities of eggs at the same cell cycle stage can be collected, packed, and broken by simple centrifugation to produce undiluted cytosol with well-preserved membrane structures. This cytosol is capable of supporting successive cycling between mitosis and interphase in vitro. Therefore, it has been used to study processes such as cell cycle regulation, DNA replication and repair, chromosome condensation and de-condensation, mitotic spindle morphogenesis, and nuclear envelope assembly. Ok, but I've heard you can't image Xenopus embryos? Actually, Xenopus is great for imaging in cell biology because the cells of the embryo are unusually big at diameters of over 30 μm. If Xenopus is so widely used, why haven't I heard of it? Well, Xenopus has diverse applications, and so people using them have tended to associate based on scientific interests rather than the organism. This has meant that Xenopus has lagged somewhat in terms of resource development, but that's changing now. Genome sequencing is underway for both X. laevis and X. tropicalis, an effort has been launched to build a Xenopus ORFeome for large-scale functional studies and Xenbase, the Xenopus model organism database, is expanding rapidly. You might not have heard about it, but there's a good chance that someone's using Xenopus just down the hall." @default.
• W4248442972 created "2022-05-12" @default.
• W4248442972 creator A5036715195 @default.
• W4248442972 creator A5048878591 @default.
• W4248442972 creator A5081016920 @default.
• W4248442972 date "2010-03-01" @default.
• W4248442972 modified "2023-10-18" @default.
• W4248442972 title "Xenopus" @default.
• W4248442972 cites W1973492470 @default.
• W4248442972 cites W1996396912 @default.
• W4248442972 cites W2023455377 @default.
• W4248442972 cites W2030322393 @default.
• W4248442972 cites W2034100797 @default.
• W4248442972 cites W2061535202 @default.
• W4248442972 cites W2076716415 @default.
• W4248442972 doi "https://doi.org/10.1016/j.cub.2010.01.012" @default.
• W4248442972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20334828" @default.
• W4248442972 hasPublicationYear "2010" @default.
• W4248442972 type Work @default.
• W4248442972 citedByCount "19" @default.
• W4248442972 countsByYear W42484429722013 @default.
• W4248442972 countsByYear W42484429722014 @default.
• W4248442972 countsByYear W42484429722015 @default.
• W4248442972 countsByYear W42484429722016 @default.
• W4248442972 countsByYear W42484429722017 @default.
• W4248442972 countsByYear W42484429722018 @default.
• W4248442972 countsByYear W42484429722019 @default.
• W4248442972 countsByYear W42484429722021 @default.
• W4248442972 countsByYear W42484429722022 @default.
• W4248442972 countsByYear W42484429722023 @default.
• W4248442972 crossrefType "journal-article" @default.
• W4248442972 hasAuthorship W4248442972A5036715195 @default.
• W4248442972 hasAuthorship W4248442972A5048878591 @default.
• W4248442972 hasAuthorship W4248442972A5081016920 @default.
• W4248442972 hasBestOaLocation W42484429721 @default.
• W4248442972 hasConcept C104317684 @default.
• W4248442972 hasConcept C18903297 @default.
• W4248442972 hasConcept C19843653 @default.
• W4248442972 hasConcept C2776718933 @default.
• W4248442972 hasConcept C2779535977 @default.
• W4248442972 hasConcept C2781465898 @default.
• W4248442972 hasConcept C54355233 @default.
• W4248442972 hasConcept C78458016 @default.
• W4248442972 hasConcept C86803240 @default.
• W4248442972 hasConcept C90856448 @default.
• W4248442972 hasConcept C95444343 @default.
• W4248442972 hasConceptScore W4248442972C104317684 @default.
• W4248442972 hasConceptScore W4248442972C18903297 @default.
• W4248442972 hasConceptScore W4248442972C19843653 @default.
• W4248442972 hasConceptScore W4248442972C2776718933 @default.
• W4248442972 hasConceptScore W4248442972C2779535977 @default.
• W4248442972 hasConceptScore W4248442972C2781465898 @default.
• W4248442972 hasConceptScore W4248442972C54355233 @default.
• W4248442972 hasConceptScore W4248442972C78458016 @default.
• W4248442972 hasConceptScore W4248442972C86803240 @default.
• W4248442972 hasConceptScore W4248442972C90856448 @default.
• W4248442972 hasConceptScore W4248442972C95444343 @default.
• W4248442972 hasIssue "6" @default.
• W4248442972 hasLocation W42484429721 @default.
• W4248442972 hasLocation W42484429722 @default.
• W4248442972 hasOpenAccess W4248442972 @default.
• W4248442972 hasPrimaryLocation W42484429721 @default.
• W4248442972 hasRelatedWork W1594627544 @default.
• W4248442972 hasRelatedWork W1986428265 @default.
• W4248442972 hasRelatedWork W2015161228 @default.
• W4248442972 hasRelatedWork W2053302830 @default.
• W4248442972 hasRelatedWork W2087000776 @default.
• W4248442972 hasRelatedWork W2115410924 @default.
• W4248442972 hasRelatedWork W2128073791 @default.
• W4248442972 hasRelatedWork W2511180745 @default.
• W4248442972 hasRelatedWork W2522345041 @default.
• W4248442972 hasRelatedWork W2527259077 @default.
• W4248442972 hasVolume "20" @default.
• W4248442972 isParatext "false" @default.
• W4248442972 isRetracted "false" @default.
• W4248442972 workType "article" @default.