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• W4248514589 abstract "Event Abstract Back to Event DNA damage in potato plants exposed to high level of NaCl stress Murat Dikilitas1*, Andrew R. Collins2, Abdurrahim Kocyigit3, Naouale EL YAMANI4 and Sema Karakas5 1 Harran University, Türkiye 2 University of Oslo, Norway 3 Bezm-i Alem Vakif University, Türkiye 4 University of Oslo, Norway 5 Harran University, Türkiye We used the alkaline protocol of the modified plant comet assay for rapid detection of DNA damage in potato plants exposed to low (50 mmol l-1), moderate (100 mmol l-1) and high (200 mmol l-1) NaCl stress in hydroponic conditions. DNA damage was measured as the values of percentage of DNA in heads and tails, and tail length. After 12 h treatments of the rooted cuttings with NaCl, a dose response increase in DNA damage was evident both in leaves and roots although the first two doses were not significantly different in leaves. Ethyl methanesulphonate was used for the comparison of induced DNA damage. When H2O2 and phenol levels were examined, a dose response accumulation of stress metabolites was evident in both plant parts although leaves accumulated to a lesser degree. Low and moderate NaCl stress have potential to create DNA damage and induced stress metabolites while high NaCl stress has devastating effects on both DNA damage and oxidative stress parameters. Correlations between parameters were also evaluated with correlation network analysis. Plants exposed to high level of NaCl stress did not recover from the effects of NaCl after 4 h recovery period in dH2O. We assume that recovery of plants from these stressful conditions depends on the level of DNA damages. Therefore, measuring DNA damage or recovery from damage would help us determine whether the organisms exposed to more than one stress factors would recover or not. References Dmitrieva, N.I., Burg, M.B. (2007). Osmotic stress and DNA damage. Methods Enzymol, 428, 241-252. Dmitrieva, N.I., Cui, K., Kitchaev, D.A., Zhao, K., Burg, M.B. (2011). DNA double-strand breaks induced by high NaCl occur predominantly in gene deserts. PNAS, 108, 20796-20801. Keywords: Comet Assay, SCGE, DNA Damage, plant, Salinity Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Poster Discussion Topic: Ecogenotoxicology Citation: Dikilitas M, Collins AR, Kocyigit A, EL YAMANI N and Karakas S (2015). DNA damage in potato plants exposed to high level of NaCl stress. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00066 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2015; Published Online: 23 Jun 2015. * Correspondence: Dr. Murat Dikilitas, Harran University, Sanliurfa, Türkiye, m.dikilitas@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers Murat Dikilitas Andrew R Collins Abdurrahim Kocyigit Naouale EL YAMANI Sema Karakas Google Murat Dikilitas Andrew R Collins Abdurrahim Kocyigit Naouale EL YAMANI Sema Karakas Google Scholar Murat Dikilitas Andrew R Collins Abdurrahim Kocyigit Naouale EL YAMANI Sema Karakas PubMed Murat Dikilitas Andrew R Collins Abdurrahim Kocyigit Naouale EL YAMANI Sema Karakas Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W4248514589 title "DNA damage in potato plants exposed to high level of NaCl stress" @default.
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