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- W4248540848 abstract "The recent development of magnets to produce a stable static field and of computers to process the data and to control the apparatus has improved the resolution of NMR spectra and the detection of NMR relaxation times. NMR is now being used as a non-destructive method for measuring substances in living tissues. This paper reports the application of NMR to living tissues, nervous tissue and muscle, together with methods of maintaining these tissues under physiological conditions during NMR measurement.The methods of maintaining tissue in the living state are: (1) perfusion of a solution into the NMR tube, (2) freezing of tissue with liquid nitrogen, and (3) insertion of living material and supplying air into the NMR tube. 31P-NMR spectra of crayfish ventral nerve cords and brain slices of guinea pig under perfusion with spinning showed the resonances of Pi, sugar phosphates and high-energy phosphorus compounds. Both frozen rat brain and the head of mouse in vivo showed almost the same 31P-NMR spectra, and the time courses of signal intensities of the phosphorus compounds in the former were obtained. 1H- and 31P-NMR were applied to frog muscle. Measurements of 1H-NMR transverse relaxation time showed that water in the muscle is composed of three fractions. 31P-NMR spectra of frog muscle at rest and after tetanus stimulation showed metabolic changes in phosphocreatine, Pi, and sugar phosphates, and changes of intracellular pH. The decrease of Pi and the increase of phosphocreatine after tetanus stimulation showed the same time course." @default.
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- W4248540848 date "1980-01-01" @default.
- W4248540848 modified "2023-09-27" @default.
- W4248540848 title "Application of NMR to Living Tissues" @default.
- W4248540848 doi "https://doi.org/10.2142/biophys.20.253" @default.
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