Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248565811> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W4248565811 endingPage "tw3" @default.
- W4248565811 startingPage "tw3" @default.
- W4248565811 abstract "Tu et al. discovered an unexpected role for synapsin Ia as an inhibitor of guanosine triphosphatase (GTPase)-activating proteins (GAPs) that stimulate Gz, a member of the Gi family of heterotrimeric guanine nucleotide-binding proteins found in neurons, platelets, and chromaffin cells. The synapsins are a family of synaptic phosphoproteins. Although their association with synaptic vesicles and the cytoskeleton has led to their implication in synaptic vesicle dynamics, other possible roles for these abundant proteins have remained undetermined. Observing that washing with high ionic strength buffer increased the Gz GAP activity of RGSZ1 in a membrane fraction from bovine brain, Tu et al. determined that a cytoskeletal or peripheral membrane protein was acting as a GAP inhibitor. After further purification, the authors isolated a high-molecular-weight GAP inhibitor that was identified as synapsin Ia by mass spectrometry, an identification confirmed by Western analysis. Recombinant rat synapsin Ia expressed in and then purified from Sf9 cells inhibited Gz GAP activity, whereas an antibody against synapsin depleted GAP-inhibitory activity from brain. Moreover, this GAP-inhibitory activity could not be isolated from the brains of mice lacking synapsin I and II. Although synapsin Ia inhibited the GAP activity of several RGS proteins, inhibition was selective for GAP activity directed at Gαz. The ability of synapsin Ia to inhibit GAP activity was attenuated by phosphorylation at Ser9 by either protein kinase A or p21-activated protein kinase. Thus, synapsin Ia appears to act as a selective Gz GAP inhibitor that is sensitive to regulation through at least two signaling pathways.Y. Tu, S. K. Nayak, J. Woodson, E. M. Ross, Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin I. J. Biol. Chem. 278, 52273-52281 (2003). [Abstract] [Full Text]" @default.
- W4248565811 created "2022-05-12" @default.
- W4248565811 date "2004-01-06" @default.
- W4248565811 modified "2023-09-26" @default.
- W4248565811 title "A Gap in Synapsin Function" @default.
- W4248565811 doi "https://doi.org/10.1126/stke.2142004tw3" @default.
- W4248565811 hasPublicationYear "2004" @default.
- W4248565811 type Work @default.
- W4248565811 citedByCount "0" @default.
- W4248565811 crossrefType "journal-article" @default.
- W4248565811 hasConcept C11960822 @default.
- W4248565811 hasConcept C130316041 @default.
- W4248565811 hasConcept C139407321 @default.
- W4248565811 hasConcept C148785051 @default.
- W4248565811 hasConcept C163181125 @default.
- W4248565811 hasConcept C26987225 @default.
- W4248565811 hasConcept C41625074 @default.
- W4248565811 hasConcept C49658373 @default.
- W4248565811 hasConcept C50493954 @default.
- W4248565811 hasConcept C55493867 @default.
- W4248565811 hasConcept C62478195 @default.
- W4248565811 hasConcept C80631254 @default.
- W4248565811 hasConcept C86803240 @default.
- W4248565811 hasConcept C95444343 @default.
- W4248565811 hasConceptScore W4248565811C11960822 @default.
- W4248565811 hasConceptScore W4248565811C130316041 @default.
- W4248565811 hasConceptScore W4248565811C139407321 @default.
- W4248565811 hasConceptScore W4248565811C148785051 @default.
- W4248565811 hasConceptScore W4248565811C163181125 @default.
- W4248565811 hasConceptScore W4248565811C26987225 @default.
- W4248565811 hasConceptScore W4248565811C41625074 @default.
- W4248565811 hasConceptScore W4248565811C49658373 @default.
- W4248565811 hasConceptScore W4248565811C50493954 @default.
- W4248565811 hasConceptScore W4248565811C55493867 @default.
- W4248565811 hasConceptScore W4248565811C62478195 @default.
- W4248565811 hasConceptScore W4248565811C80631254 @default.
- W4248565811 hasConceptScore W4248565811C86803240 @default.
- W4248565811 hasConceptScore W4248565811C95444343 @default.
- W4248565811 hasIssue "214" @default.
- W4248565811 hasLocation W42485658111 @default.
- W4248565811 hasOpenAccess W4248565811 @default.
- W4248565811 hasPrimaryLocation W42485658111 @default.
- W4248565811 hasRelatedWork W1481610803 @default.
- W4248565811 hasRelatedWork W1564969903 @default.
- W4248565811 hasRelatedWork W1599069035 @default.
- W4248565811 hasRelatedWork W2035584793 @default.
- W4248565811 hasRelatedWork W2052622089 @default.
- W4248565811 hasRelatedWork W2054496958 @default.
- W4248565811 hasRelatedWork W2094565395 @default.
- W4248565811 hasRelatedWork W2099510312 @default.
- W4248565811 hasRelatedWork W2148897550 @default.
- W4248565811 hasRelatedWork W2626827638 @default.
- W4248565811 hasVolume "2004" @default.
- W4248565811 isParatext "false" @default.
- W4248565811 isRetracted "false" @default.
- W4248565811 workType "article" @default.