Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248620006> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W4248620006 abstract "Abstract Background Neural cell adhesion molecule (NCAM), a common mammalian cell surface glycoprotein, is the major substrate of polysialic acid (polySia). Polysialylated NCAM occurs in many types of cancer, but rarely in normal adult tissues. Epithelial-mesenchymal transition (EMT) is an important process contributing to tumor metastasis. The functional role of NCAM hypersialylation in EMT is unclear. Method Expression of NCAM and polysialylated NCAM in breast cancer progression were evaluated by western blot (WB), immunohistochemistry, semi-quantitative PCR and immunoprecipitation (IP). Overexpression of NCAM-140 and ST8SiaII were performed to assess the functional role of NCAM hypersialylation by liposome transfection. Cell proliferation ability was investigated with MTT assay. Transwell and wound closure assay were conducted to evaluate cell migratory ability. Phagokinetic gold sol assay and cell adhesion assay were performed to assess cell motility ability and cell adhesion ability, respectively. Furthermore, WB and IP were used to reveal the activated signaling pathway. Results The present study indicates that NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways, based on the following observations: (i) NCAM and polysialylated NCAM were aberrantly regulated in breast cancer cells; (ii) NCAM and polysialylated NCAM expression were upregulated in normal breast epithelial cells undergoing EMT; (iii) NCAM overexpression induced EMT in breast epithelial cells; (iv) NCAM promoted cell proliferation and migration through activation of a β-catenin/slug signaling pathway; (v) modification of polySia attached to NCAM inhibited cell adhesion and promoted cell motility through activation of an EGFR/STAT3 pathway.Conclusion This study demonstrates that NCAM and polysialylated NCAM facilitate different signaling pathway and affect different cell behaviors. Switching between the NCAM-mediated pathways appeared to depend on polySia decoration." @default.
- W4248620006 created "2022-05-12" @default.
- W4248620006 creator A5005390562 @default.
- W4248620006 creator A5006822602 @default.
- W4248620006 creator A5012088653 @default.
- W4248620006 creator A5012516044 @default.
- W4248620006 creator A5013062318 @default.
- W4248620006 creator A5015355620 @default.
- W4248620006 creator A5053693099 @default.
- W4248620006 creator A5081940891 @default.
- W4248620006 date "2020-04-27" @default.
- W4248620006 modified "2023-10-16" @default.
- W4248620006 title "NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways" @default.
- W4248620006 doi "https://doi.org/10.21203/rs.2.24259/v3" @default.
- W4248620006 hasPublicationYear "2020" @default.
- W4248620006 type Work @default.
- W4248620006 citedByCount "0" @default.
- W4248620006 crossrefType "posted-content" @default.
- W4248620006 hasAuthorship W4248620006A5005390562 @default.
- W4248620006 hasAuthorship W4248620006A5006822602 @default.
- W4248620006 hasAuthorship W4248620006A5012088653 @default.
- W4248620006 hasAuthorship W4248620006A5012516044 @default.
- W4248620006 hasAuthorship W4248620006A5013062318 @default.
- W4248620006 hasAuthorship W4248620006A5015355620 @default.
- W4248620006 hasAuthorship W4248620006A5053693099 @default.
- W4248620006 hasAuthorship W4248620006A5081940891 @default.
- W4248620006 hasBestOaLocation W42486200061 @default.
- W4248620006 hasConcept C122871604 @default.
- W4248620006 hasConcept C137738243 @default.
- W4248620006 hasConcept C1491633281 @default.
- W4248620006 hasConcept C185592680 @default.
- W4248620006 hasConcept C2778993516 @default.
- W4248620006 hasConcept C502942594 @default.
- W4248620006 hasConcept C55493867 @default.
- W4248620006 hasConcept C62478195 @default.
- W4248620006 hasConcept C85789140 @default.
- W4248620006 hasConcept C86803240 @default.
- W4248620006 hasConcept C95444343 @default.
- W4248620006 hasConceptScore W4248620006C122871604 @default.
- W4248620006 hasConceptScore W4248620006C137738243 @default.
- W4248620006 hasConceptScore W4248620006C1491633281 @default.
- W4248620006 hasConceptScore W4248620006C185592680 @default.
- W4248620006 hasConceptScore W4248620006C2778993516 @default.
- W4248620006 hasConceptScore W4248620006C502942594 @default.
- W4248620006 hasConceptScore W4248620006C55493867 @default.
- W4248620006 hasConceptScore W4248620006C62478195 @default.
- W4248620006 hasConceptScore W4248620006C85789140 @default.
- W4248620006 hasConceptScore W4248620006C86803240 @default.
- W4248620006 hasConceptScore W4248620006C95444343 @default.
- W4248620006 hasLocation W42486200061 @default.
- W4248620006 hasLocation W42486200062 @default.
- W4248620006 hasOpenAccess W4248620006 @default.
- W4248620006 hasPrimaryLocation W42486200061 @default.
- W4248620006 hasRelatedWork W12474162 @default.
- W4248620006 hasRelatedWork W12579472 @default.
- W4248620006 hasRelatedWork W15624534 @default.
- W4248620006 hasRelatedWork W15754500 @default.
- W4248620006 hasRelatedWork W18145972 @default.
- W4248620006 hasRelatedWork W2851962 @default.
- W4248620006 hasRelatedWork W3459498 @default.
- W4248620006 hasRelatedWork W4431115 @default.
- W4248620006 hasRelatedWork W5322158 @default.
- W4248620006 hasRelatedWork W9739135 @default.
- W4248620006 isParatext "false" @default.
- W4248620006 isRetracted "false" @default.
- W4248620006 workType "article" @default.