Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248687002> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W4248687002 abstract "Abstract Ancient DNA analysis of human oral microbial communities within calcified dental plaque (calculus) has revealed key insights into human health, paleodemography, and cultural behaviors. However, contamination imposes a major concern for paleomicrobiological samples due to their low endogenous DNA content and exposure to environmental sources, calling into question some published results. Decontamination protocols ( e.g. an ethylenediaminetetraacetic acid (EDTA) pre-digestion or ultraviolet radiation (UV) and bleach immersion treatments) aim to minimize the exogenous content of the outer surface of ancient calculus samples prior to DNA extraction. While these protocols are efficient, no one has systematically compared them. Here, we compare untreated dental calculus samples to four previously published decontamination protocols: a UV only treatment; a 5% sodium hypochlorite (bleach) immersion treatment; a pre-digestion in EDTA treatment; and a combined UV irradiation and 5% bleach immersion treatment. We examine their efficacy in ancient oral microbiota recovery by applying 16S rRNA gene amplicon and shotgun sequencing to ancient calculus samples from a single site. We identify ancient oral microbiota, as well as soil and skin contaminants. Overall, both the EDTA and UVB treatments are effective at reducing the proportion of environmental taxa while increasing oral taxa in relation to untreated samples." @default.
- W4248687002 created "2022-05-12" @default.
- W4248687002 creator A5017886800 @default.
- W4248687002 creator A5056014770 @default.
- W4248687002 creator A5062689955 @default.
- W4248687002 creator A5068031607 @default.
- W4248687002 creator A5086641671 @default.
- W4248687002 creator A5091096749 @default.
- W4248687002 date "2020-12-31" @default.
- W4248687002 modified "2023-09-27" @default.
- W4248687002 title "Effectiveness of Decontamination protocols when Analyzing Ancient DNA Preserved in Dental Calculus" @default.
- W4248687002 doi "https://doi.org/10.21203/rs.3.rs-136836/v1" @default.
- W4248687002 hasPublicationYear "2020" @default.
- W4248687002 type Work @default.
- W4248687002 citedByCount "0" @default.
- W4248687002 crossrefType "posted-content" @default.
- W4248687002 hasAuthorship W4248687002A5017886800 @default.
- W4248687002 hasAuthorship W4248687002A5056014770 @default.
- W4248687002 hasAuthorship W4248687002A5062689955 @default.
- W4248687002 hasAuthorship W4248687002A5068031607 @default.
- W4248687002 hasAuthorship W4248687002A5086641671 @default.
- W4248687002 hasAuthorship W4248687002A5091096749 @default.
- W4248687002 hasBestOaLocation W42486870021 @default.
- W4248687002 hasConcept C107211472 @default.
- W4248687002 hasConcept C112570922 @default.
- W4248687002 hasConcept C142724271 @default.
- W4248687002 hasConcept C158689903 @default.
- W4248687002 hasConcept C178790620 @default.
- W4248687002 hasConcept C185592680 @default.
- W4248687002 hasConcept C18903297 @default.
- W4248687002 hasConcept C189338579 @default.
- W4248687002 hasConcept C197404232 @default.
- W4248687002 hasConcept C199343813 @default.
- W4248687002 hasConcept C2777686260 @default.
- W4248687002 hasConcept C2777966455 @default.
- W4248687002 hasConcept C2778973965 @default.
- W4248687002 hasConcept C2908647359 @default.
- W4248687002 hasConcept C523546767 @default.
- W4248687002 hasConcept C54355233 @default.
- W4248687002 hasConcept C71924100 @default.
- W4248687002 hasConcept C86803240 @default.
- W4248687002 hasConcept C99454951 @default.
- W4248687002 hasConceptScore W4248687002C107211472 @default.
- W4248687002 hasConceptScore W4248687002C112570922 @default.
- W4248687002 hasConceptScore W4248687002C142724271 @default.
- W4248687002 hasConceptScore W4248687002C158689903 @default.
- W4248687002 hasConceptScore W4248687002C178790620 @default.
- W4248687002 hasConceptScore W4248687002C185592680 @default.
- W4248687002 hasConceptScore W4248687002C18903297 @default.
- W4248687002 hasConceptScore W4248687002C189338579 @default.
- W4248687002 hasConceptScore W4248687002C197404232 @default.
- W4248687002 hasConceptScore W4248687002C199343813 @default.
- W4248687002 hasConceptScore W4248687002C2777686260 @default.
- W4248687002 hasConceptScore W4248687002C2777966455 @default.
- W4248687002 hasConceptScore W4248687002C2778973965 @default.
- W4248687002 hasConceptScore W4248687002C2908647359 @default.
- W4248687002 hasConceptScore W4248687002C523546767 @default.
- W4248687002 hasConceptScore W4248687002C54355233 @default.
- W4248687002 hasConceptScore W4248687002C71924100 @default.
- W4248687002 hasConceptScore W4248687002C86803240 @default.
- W4248687002 hasConceptScore W4248687002C99454951 @default.
- W4248687002 hasLocation W42486870021 @default.
- W4248687002 hasLocation W42486870022 @default.
- W4248687002 hasLocation W42486870023 @default.
- W4248687002 hasOpenAccess W4248687002 @default.
- W4248687002 hasPrimaryLocation W42486870021 @default.
- W4248687002 hasRelatedWork W1487860174 @default.
- W4248687002 hasRelatedWork W2078568644 @default.
- W4248687002 hasRelatedWork W2166642037 @default.
- W4248687002 hasRelatedWork W2334385784 @default.
- W4248687002 hasRelatedWork W2756384320 @default.
- W4248687002 hasRelatedWork W2921705712 @default.
- W4248687002 hasRelatedWork W2973203446 @default.
- W4248687002 hasRelatedWork W3113968870 @default.
- W4248687002 hasRelatedWork W4248687002 @default.
- W4248687002 hasRelatedWork W2786466323 @default.
- W4248687002 isParatext "false" @default.
- W4248687002 isRetracted "false" @default.
- W4248687002 workType "article" @default.