FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248862391> ?p ?o ?g. }

• W4248862391 endingPage "255" @default.
• W4248862391 startingPage "250" @default.
• W4248862391 abstract "Platelet activation results in changes in a number of cell surface molecules including an increase in P-Selectin (CD62P) that may be rapidly and conveniently measured by immunofluorescent flow cytometry. The ADVIA 120 (Bayer) is a new system that facilitates more accurate measurement of platelet volume and in addition provides an approximate measure of the mean refractive index (RI) of the platelets reported as mean platelet component (MPC) concentration. We were interested to determine whether changes in MPC might reflect changes in platelet activation status. To investigate this, the platelet CD62P expression, determined by flow cytometry, and change in MPC, measured on the ADVIA 120 system, was first examined in vitro after stimulation of EDTA anticoagulated whole blood with submaximal concentrations of bovine thrombin in the presence or absence of the thromboxane synthase inhibitor, Ridogrel. Thrombin produced a dose-dependent increase in platelet CD62P expression and a decrease in MPC that could be inhibited by Ridogrel at physiological concentrations. In the second set of experiments, blood from 20 normal controls was collected into both EDTA and sodium citrate (SC) anticoagulants. Within 30 min of venesection and again at 3 h post-venesection after storage at room temperature, the platelet MPC and CD62P expression were determined. Platelets in all samples with both anticoagulants showed very low levels of CD62P expression when first analysed. At 3 h there was a small increase in CD62P expression on platelets in whole blood anticoagulated with SC, but a significant (P < 0.001) increase was observed on platelets anti-coagulated with EDTA. A negative correlation was found between the change in MPC of the platelets and the increase in the mean fluorescence intensity (MFI) (r = −0.69, P < 0.001, n = 20) and the percentage (r = −0.72, P < 0.001, n = 20) of CD62P positive platelets at 3 h in blood anticoagulated with EDTA. We conclude that a reduction in MPC as measured by the ADVIA 120 may be used to detect anticoagulant induced, as well as thrombin stimulated, in vitro platelet activation in blood anticoagulated with EDTA. Further, we conclude that platelet activation is negligible for up to 3 h in sodium citrate anticoagulated whole blood. Cytometry (Comm. Clin. Cytometry) 38:250–255, 1999. © 1999 Wiley-Liss, Inc." @default.
• W4248862391 created "2022-05-12" @default.
• W4248862391 creator A5008874330 @default.
• W4248862391 creator A5011059993 @default.
• W4248862391 creator A5012770387 @default.
• W4248862391 creator A5013102182 @default.
• W4248862391 creator A5048427136 @default.
• W4248862391 creator A5067725332 @default.
• W4248862391 creator A5078453455 @default.
• W4248862391 creator A5081446369 @default.
• W4248862391 date "1999-10-15" @default.
• W4248862391 modified "2023-09-30" @default.
• W4248862391 title "Use of mean platelet component to measure platelet activation on the ADVIA 120 haematology system" @default.
• W4248862391 cites W1559985172 @default.
• W4248862391 cites W1965526093 @default.
• W4248862391 cites W1976558714 @default.
• W4248862391 cites W1977070858 @default.
• W4248862391 cites W1980099544 @default.
• W4248862391 cites W1985321440 @default.
• W4248862391 cites W1987016224 @default.
• W4248862391 cites W1990482351 @default.
• W4248862391 cites W1992914829 @default.
• W4248862391 cites W1996263109 @default.
• W4248862391 cites W1996715488 @default.
• W4248862391 cites W2012285989 @default.
• W4248862391 cites W2013460120 @default.
• W4248862391 cites W2019984160 @default.
• W4248862391 cites W2035369939 @default.
• W4248862391 cites W2043503971 @default.
• W4248862391 cites W2057714798 @default.
• W4248862391 cites W2069348092 @default.
• W4248862391 cites W2072009136 @default.
• W4248862391 cites W2083571225 @default.
• W4248862391 cites W2092672624 @default.
• W4248862391 cites W2123490492 @default.
• W4248862391 cites W2143317626 @default.
• W4248862391 cites W2314766304 @default.
• W4248862391 cites W2327484480 @default.
• W4248862391 cites W2461339661 @default.
• W4248862391 cites W4239471829 @default.
• W4248862391 doi "https://doi.org/10.1002/(sici)1097-0320(19991015)38:5<250::aid-cyto8>3.0.co;2-k" @default.
• W4248862391 hasPublicationYear "1999" @default.
• W4248862391 type Work @default.
• W4248862391 citedByCount "72" @default.
• W4248862391 countsByYear W42488623912012 @default.
• W4248862391 countsByYear W42488623912013 @default.
• W4248862391 countsByYear W42488623912014 @default.
• W4248862391 countsByYear W42488623912015 @default.
• W4248862391 countsByYear W42488623912016 @default.
• W4248862391 countsByYear W42488623912017 @default.
• W4248862391 countsByYear W42488623912018 @default.
• W4248862391 countsByYear W42488623912019 @default.
• W4248862391 countsByYear W42488623912020 @default.
• W4248862391 countsByYear W42488623912021 @default.
• W4248862391 countsByYear W42488623912022 @default.
• W4248862391 countsByYear W42488623912023 @default.
• W4248862391 crossrefType "journal-article" @default.
• W4248862391 hasAuthorship W4248862391A5008874330 @default.
• W4248862391 hasAuthorship W4248862391A5011059993 @default.
• W4248862391 hasAuthorship W4248862391A5012770387 @default.
• W4248862391 hasAuthorship W4248862391A5013102182 @default.
• W4248862391 hasAuthorship W4248862391A5048427136 @default.
• W4248862391 hasAuthorship W4248862391A5067725332 @default.
• W4248862391 hasAuthorship W4248862391A5078453455 @default.
• W4248862391 hasAuthorship W4248862391A5081446369 @default.
• W4248862391 hasBestOaLocation W42488623911 @default.
• W4248862391 hasConcept C126322002 @default.
• W4248862391 hasConcept C142724271 @default.
• W4248862391 hasConcept C1621761 @default.
• W4248862391 hasConcept C16685009 @default.
• W4248862391 hasConcept C185592680 @default.
• W4248862391 hasConcept C190481736 @default.
• W4248862391 hasConcept C203014093 @default.
• W4248862391 hasConcept C2777292125 @default.
• W4248862391 hasConcept C2780742167 @default.
• W4248862391 hasConcept C3018697912 @default.
• W4248862391 hasConcept C553184892 @default.
• W4248862391 hasConcept C56701166 @default.
• W4248862391 hasConcept C71924100 @default.
• W4248862391 hasConcept C89560881 @default.
• W4248862391 hasConceptScore W4248862391C126322002 @default.
• W4248862391 hasConceptScore W4248862391C142724271 @default.
• W4248862391 hasConceptScore W4248862391C1621761 @default.
• W4248862391 hasConceptScore W4248862391C16685009 @default.
• W4248862391 hasConceptScore W4248862391C185592680 @default.
• W4248862391 hasConceptScore W4248862391C190481736 @default.
• W4248862391 hasConceptScore W4248862391C203014093 @default.
• W4248862391 hasConceptScore W4248862391C2777292125 @default.
• W4248862391 hasConceptScore W4248862391C2780742167 @default.
• W4248862391 hasConceptScore W4248862391C3018697912 @default.
• W4248862391 hasConceptScore W4248862391C553184892 @default.
• W4248862391 hasConceptScore W4248862391C56701166 @default.
• W4248862391 hasConceptScore W4248862391C71924100 @default.
• W4248862391 hasConceptScore W4248862391C89560881 @default.
• W4248862391 hasIssue "5" @default.
• W4248862391 hasLocation W42488623911 @default.
• W4248862391 hasOpenAccess W4248862391 @default.
• W4248862391 hasPrimaryLocation W42488623911 @default.

• next