Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248929097> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W4248929097 endingPage "267" @default.
- W4248929097 startingPage "231" @default.
- W4248929097 abstract "In the previous chapter we demonstrated the power of mass spectrometry for the study of biomolecules at equilibrium. For instance we can allow a sample to equilibrate in the solution conditions of choice and then acquire a spectrum at leisure. By varying the solvent composition it becomes possible to infer some of the properties of alternate conformations transiently sampled in the equilibrium population, yielding information about the structural features of the states that are visited. Analysis of charge state distributions exhibited in ESI mass spectra provides valuable information about the degree of “foldedness” of these conformational ensembles, and from hydrogen exchange experiments it is possible to measure the exposure to solvent of these species. Kinetic studies are crucial to a complete understanding of biochemical reaction mechanisms, including protein folding. Kinetic investigations enable rate constants and activation energies to be derived, and by monitoring the progress of the reaction it may be possible to observe transient intermediates that give key mechanistic information. Transient intermediates detected during protein folding can provide insight into the dominant pathway of folding; likewise short lived intermediate species during the catalytic cycle of enzyme activity gives valuable information about the reaction mechanism. However these intermediates are, by their very nature, short lived so, unlike equilibrium measurements, there is the added challenge of time scale. Once a reaction is initiated it must be rapidly transferred to an observation point in order to be followed kinetically. In this chapter we will discuss some of the methodologies developed to study these kinetic events." @default.
- W4248929097 created "2022-05-12" @default.
- W4248929097 date "2005-01-27" @default.
- W4248929097 modified "2023-10-18" @default.
- W4248929097 title "Kinetic Studies by Mass Spectrometry" @default.
- W4248929097 doi "https://doi.org/10.1002/0471705179.ch6" @default.
- W4248929097 hasPublicationYear "2005" @default.
- W4248929097 type Work @default.
- W4248929097 citedByCount "1" @default.
- W4248929097 crossrefType "other" @default.
- W4248929097 hasConcept C119599485 @default.
- W4248929097 hasConcept C121332964 @default.
- W4248929097 hasConcept C127413603 @default.
- W4248929097 hasConcept C135889238 @default.
- W4248929097 hasConcept C144024400 @default.
- W4248929097 hasConcept C147597530 @default.
- W4248929097 hasConcept C148898269 @default.
- W4248929097 hasConcept C149923435 @default.
- W4248929097 hasConcept C159467904 @default.
- W4248929097 hasConcept C162356407 @default.
- W4248929097 hasConcept C178790620 @default.
- W4248929097 hasConcept C185592680 @default.
- W4248929097 hasConcept C204328495 @default.
- W4248929097 hasConcept C2776545253 @default.
- W4248929097 hasConcept C2780471494 @default.
- W4248929097 hasConcept C2908647359 @default.
- W4248929097 hasConcept C43617362 @default.
- W4248929097 hasConcept C49853544 @default.
- W4248929097 hasConcept C55493867 @default.
- W4248929097 hasConcept C62520636 @default.
- W4248929097 hasConcept C93391505 @default.
- W4248929097 hasConceptScore W4248929097C119599485 @default.
- W4248929097 hasConceptScore W4248929097C121332964 @default.
- W4248929097 hasConceptScore W4248929097C127413603 @default.
- W4248929097 hasConceptScore W4248929097C135889238 @default.
- W4248929097 hasConceptScore W4248929097C144024400 @default.
- W4248929097 hasConceptScore W4248929097C147597530 @default.
- W4248929097 hasConceptScore W4248929097C148898269 @default.
- W4248929097 hasConceptScore W4248929097C149923435 @default.
- W4248929097 hasConceptScore W4248929097C159467904 @default.
- W4248929097 hasConceptScore W4248929097C162356407 @default.
- W4248929097 hasConceptScore W4248929097C178790620 @default.
- W4248929097 hasConceptScore W4248929097C185592680 @default.
- W4248929097 hasConceptScore W4248929097C204328495 @default.
- W4248929097 hasConceptScore W4248929097C2776545253 @default.
- W4248929097 hasConceptScore W4248929097C2780471494 @default.
- W4248929097 hasConceptScore W4248929097C2908647359 @default.
- W4248929097 hasConceptScore W4248929097C43617362 @default.
- W4248929097 hasConceptScore W4248929097C49853544 @default.
- W4248929097 hasConceptScore W4248929097C55493867 @default.
- W4248929097 hasConceptScore W4248929097C62520636 @default.
- W4248929097 hasConceptScore W4248929097C93391505 @default.
- W4248929097 hasLocation W42489290971 @default.
- W4248929097 hasOpenAccess W4248929097 @default.
- W4248929097 hasPrimaryLocation W42489290971 @default.
- W4248929097 hasRelatedWork W1980793472 @default.
- W4248929097 hasRelatedWork W1991433153 @default.
- W4248929097 hasRelatedWork W2015321541 @default.
- W4248929097 hasRelatedWork W2021456587 @default.
- W4248929097 hasRelatedWork W2031581952 @default.
- W4248929097 hasRelatedWork W2039077224 @default.
- W4248929097 hasRelatedWork W2084642783 @default.
- W4248929097 hasRelatedWork W2087321793 @default.
- W4248929097 hasRelatedWork W2089397569 @default.
- W4248929097 hasRelatedWork W2170846952 @default.
- W4248929097 isParatext "false" @default.
- W4248929097 isRetracted "false" @default.
- W4248929097 workType "other" @default.