Matches in SemOpenAlex for { <https://semopenalex.org/work/W4248972495> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4248972495 endingPage "274" @default.
- W4248972495 startingPage "273" @default.
- W4248972495 abstract "The intracellular pH (pHin) of Saccharomyces cerevisiae was measured employing fluorescence ratio imaging microscopy (FRIM). The yeast cells were fluorescently labeled with the pH dependent probe 5(and-6)-carboxyfluorescein (cF) or 5(and-6)-carboxyfluorescein succinimidyl ester (cFSE), and subsequently attached to ferric nitrate pretreated glass slides. The labeled and adhered cells could still divide and were metabolically active. Measurement of the pHin was performed during continuous perfusion of the cells with buffer or medium. Cells labeled with cF are highly fluorescent and in non-energized cells the pHin could be easily measured. However, in energized yeast cells cF was accumulated in the vacuoles and/or exported to the extracellular environment, most likely by an energy-dependent transport system, thus limiting the time period over which the pHin can be effectively measured. Therefore, cFSE (which conjugates with aliphatic amines in the cytoplasm) was applied to prevent translocation of fluorescent probe to the vacuole and/or extracellular environment. The continuous perfusion in combination with the cFSE labeling of the immobilized cells was successfully applied to determine the effect of low and high pH, and addition of glucose on the pHin of individual yeast cells over a long time period." @default.
- W4248972495 created "2022-05-12" @default.
- W4248972495 date "2000-02-01" @default.
- W4248972495 modified "2023-10-16" @default.
- W4248972495 title "Index" @default.
- W4248972495 doi "https://doi.org/10.1016/s0167-7012(99)00140-2" @default.
- W4248972495 hasPublicationYear "2000" @default.
- W4248972495 type Work @default.
- W4248972495 citedByCount "0" @default.
- W4248972495 crossrefType "other" @default.
- W4248972495 hasConcept C102568950 @default.
- W4248972495 hasConcept C121332964 @default.
- W4248972495 hasConcept C12554922 @default.
- W4248972495 hasConcept C134897140 @default.
- W4248972495 hasConcept C169274487 @default.
- W4248972495 hasConcept C181199279 @default.
- W4248972495 hasConcept C185592680 @default.
- W4248972495 hasConcept C190062978 @default.
- W4248972495 hasConcept C2777576037 @default.
- W4248972495 hasConcept C2779222958 @default.
- W4248972495 hasConcept C28406088 @default.
- W4248972495 hasConcept C55493867 @default.
- W4248972495 hasConcept C62520636 @default.
- W4248972495 hasConcept C79879829 @default.
- W4248972495 hasConcept C86803240 @default.
- W4248972495 hasConcept C91881484 @default.
- W4248972495 hasConcept C98539663 @default.
- W4248972495 hasConceptScore W4248972495C102568950 @default.
- W4248972495 hasConceptScore W4248972495C121332964 @default.
- W4248972495 hasConceptScore W4248972495C12554922 @default.
- W4248972495 hasConceptScore W4248972495C134897140 @default.
- W4248972495 hasConceptScore W4248972495C169274487 @default.
- W4248972495 hasConceptScore W4248972495C181199279 @default.
- W4248972495 hasConceptScore W4248972495C185592680 @default.
- W4248972495 hasConceptScore W4248972495C190062978 @default.
- W4248972495 hasConceptScore W4248972495C2777576037 @default.
- W4248972495 hasConceptScore W4248972495C2779222958 @default.
- W4248972495 hasConceptScore W4248972495C28406088 @default.
- W4248972495 hasConceptScore W4248972495C55493867 @default.
- W4248972495 hasConceptScore W4248972495C62520636 @default.
- W4248972495 hasConceptScore W4248972495C79879829 @default.
- W4248972495 hasConceptScore W4248972495C86803240 @default.
- W4248972495 hasConceptScore W4248972495C91881484 @default.
- W4248972495 hasConceptScore W4248972495C98539663 @default.
- W4248972495 hasIssue "3" @default.
- W4248972495 hasLocation W42489724951 @default.
- W4248972495 hasOpenAccess W4248972495 @default.
- W4248972495 hasPrimaryLocation W42489724951 @default.
- W4248972495 hasRelatedWork W1865230559 @default.
- W4248972495 hasRelatedWork W1969394390 @default.
- W4248972495 hasRelatedWork W2045326152 @default.
- W4248972495 hasRelatedWork W2061001841 @default.
- W4248972495 hasRelatedWork W2147123024 @default.
- W4248972495 hasRelatedWork W2339822182 @default.
- W4248972495 hasRelatedWork W3040767032 @default.
- W4248972495 hasRelatedWork W3132007339 @default.
- W4248972495 hasRelatedWork W3183158762 @default.
- W4248972495 hasRelatedWork W4244664902 @default.
- W4248972495 hasVolume "39" @default.
- W4248972495 isParatext "true" @default.
- W4248972495 isRetracted "false" @default.
- W4248972495 workType "paratext" @default.