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- W4248976191 abstract "The potential utility of capillary electrophoresis (CE) as an analysis and biological assay method for rapid screening of single-component combinatory libraries is demonstrated by mimicking a 5×5 drug matrix. Twenty-five compounds (some drugs, vitamins, metabolites, etc.) that include biotin are selected as the test analytes and avidin is treated as the biological activity screening reagent. In order to rapidly screen the compounds, five mixtures of nine components each are created at the diagonal of the matrix by pooling each component from the same column and row. The separation power of CE and related techniques is such that the components in these randomly generated nine-compound mixtures are almost always resolved using a single logical set of separation conditions. After obtaining optimum separation conditions, affinity testing produces the locations of the possible active component(s) based on changes in peak area. Blind tests are conducted that successfully locate the active component(s) of the matrix. With each test requiring about 14 separations (7 with and 7 without avidin) and each separation within 15 min, we were able to analyze the mixture and identify the exact locations of two possible active compounds (biotin and an unexpected compound) in the 5×5 matrix. The effect of avidin concentration on the screening procedure is assessed. Another protein, myglobin, was tested to compare specific and nonspecific interactions and to assign a threshold value (percent decrease in peak area) for determining a positive affinity response. © 1998 John Wiley & Sons, Inc. J Micro Sep 10: 653–660, 1998" @default.
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- W4248976191 date "1998-01-01" @default.
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- W4248976191 title "Evaluation of a capillary electrophoretic method for rapid screening of single‐component combinatory libraries" @default.
- W4248976191 doi "https://doi.org/10.1002/(sici)1520-667x(1998)10:8<653::aid-mcs4>3.3.co;2-3" @default.
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