Matches in SemOpenAlex for { <https://semopenalex.org/work/W4249246112> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W4249246112 abstract "Abstract Background The detection and dissection of epidermal subgroups could lead to an improved understanding of skin homeostasis and wound healing. Flow cytometric analysis provides an effective method to detect the surface markers of epidermal cells while producing high-dimensional data files. Methods A 9-color flow cytometric panel was optimized to reveal the heterogeneous subgroups in the epidermis of human skin. The subsets of epidermal cells were characterized using automated methods based on dimensional reduction approaches (viSNE) and clustering with Spanning-tree Progression Analysis of Density-normalized Events (SPADE). Results The manual analysis revealed differences in epidermal distribution between body sites based on a series biaxial gating starting with the expression of CD49f and CD29. The computational analysis divided the whole epidermal cell population into 25 clusters according to the surface marker phenotype with SPADE. This automatic analysis delineated the differences between body sites. The consistency of the results was confirmed with PhenoGraph. Conclusion A multicolor flow cytometry panel with a streamlined computational analysis pipeline is a feasible approach to delineate the heterogeneity of the epidermis in human skin." @default.
- W4249246112 created "2022-05-12" @default.
- W4249246112 creator A5008337573 @default.
- W4249246112 creator A5012631132 @default.
- W4249246112 creator A5029892501 @default.
- W4249246112 creator A5031202180 @default.
- W4249246112 creator A5047947116 @default.
- W4249246112 creator A5048639910 @default.
- W4249246112 creator A5072397178 @default.
- W4249246112 date "2021-02-12" @default.
- W4249246112 modified "2023-09-29" @default.
- W4249246112 title "Computational Flow Cytometric Analysis to Detect Epidermal Subpopulations In Human Skin" @default.
- W4249246112 doi "https://doi.org/10.21203/rs.3.rs-101283/v2" @default.
- W4249246112 hasPublicationYear "2021" @default.
- W4249246112 type Work @default.
- W4249246112 citedByCount "0" @default.
- W4249246112 crossrefType "posted-content" @default.
- W4249246112 hasAuthorship W4249246112A5008337573 @default.
- W4249246112 hasAuthorship W4249246112A5012631132 @default.
- W4249246112 hasAuthorship W4249246112A5029892501 @default.
- W4249246112 hasAuthorship W4249246112A5031202180 @default.
- W4249246112 hasAuthorship W4249246112A5047947116 @default.
- W4249246112 hasAuthorship W4249246112A5048639910 @default.
- W4249246112 hasAuthorship W4249246112A5072397178 @default.
- W4249246112 hasBestOaLocation W42492461121 @default.
- W4249246112 hasConcept C105702510 @default.
- W4249246112 hasConcept C153911025 @default.
- W4249246112 hasConcept C2776458125 @default.
- W4249246112 hasConcept C2777459323 @default.
- W4249246112 hasConcept C2908647359 @default.
- W4249246112 hasConcept C54355233 @default.
- W4249246112 hasConcept C553184892 @default.
- W4249246112 hasConcept C71924100 @default.
- W4249246112 hasConcept C86803240 @default.
- W4249246112 hasConcept C99454951 @default.
- W4249246112 hasConceptScore W4249246112C105702510 @default.
- W4249246112 hasConceptScore W4249246112C153911025 @default.
- W4249246112 hasConceptScore W4249246112C2776458125 @default.
- W4249246112 hasConceptScore W4249246112C2777459323 @default.
- W4249246112 hasConceptScore W4249246112C2908647359 @default.
- W4249246112 hasConceptScore W4249246112C54355233 @default.
- W4249246112 hasConceptScore W4249246112C553184892 @default.
- W4249246112 hasConceptScore W4249246112C71924100 @default.
- W4249246112 hasConceptScore W4249246112C86803240 @default.
- W4249246112 hasConceptScore W4249246112C99454951 @default.
- W4249246112 hasLocation W42492461121 @default.
- W4249246112 hasLocation W42492461122 @default.
- W4249246112 hasOpenAccess W4249246112 @default.
- W4249246112 hasPrimaryLocation W42492461121 @default.
- W4249246112 hasRelatedWork W11803546 @default.
- W4249246112 hasRelatedWork W12414176 @default.
- W4249246112 hasRelatedWork W17671547 @default.
- W4249246112 hasRelatedWork W27185518 @default.
- W4249246112 hasRelatedWork W31871113 @default.
- W4249246112 hasRelatedWork W35032427 @default.
- W4249246112 hasRelatedWork W37614396 @default.
- W4249246112 hasRelatedWork W6535195 @default.
- W4249246112 hasRelatedWork W6632255 @default.
- W4249246112 hasRelatedWork W6875991 @default.
- W4249246112 isParatext "false" @default.
- W4249246112 isRetracted "false" @default.
- W4249246112 workType "article" @default.