Matches in SemOpenAlex for { <https://semopenalex.org/work/W4249281569> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4249281569 endingPage "7544" @default.
- W4249281569 startingPage "7532" @default.
- W4249281569 abstract "The intracellular concentration of the microsomal triglyceride transfer protein large subunit (lMTP), the abetalipoproteinemia gene product, is tightly controlled. To date, attempts at overexpressing lMTP in vivo or in vitro have been unsuccessful. We successfully overexpressed lMTP in HepG2 cells using an adenoviral vector containing an lMTP cDNA, AdMTP. AdMTP-transduced HepG2 cells overexpressed MTP activity. They secreted increased amounts of apoB-100 lipoproteins with LDL and HDL density into the medium. lMTP overexpression alone minimally changed the density profile of apoB-containing lipoproteins, but addition of oleic acid shifted the profile toward lower densities. Oleic acid had a greater stimulatory effect on apoB-100 secretion in control HepG2 cells than in AdMTP-transduced cells, because (i) adenoviral transduction per se suppressed protein synthesis, affecting apoB-100 and albumin equally, and (ii) adenoviral transduction partially attenuated the increase in triglyceride synthesis in response to oleic acid supplementation. AdMTP treatment greatly diminished the intracellular degradation of apoB-100, but in comparison with recombinant virus containing luciferase cDNA (AdLuc), it caused no change in its biosynthetic rate. It greatly reduced, but did not eliminate, its proteasomal degradation. Our study constitutes the initial demonstration that adenovirus-mediated transfer of lMTP markedly stimulates MTP expression which in turn stimulates apoB-100 production. The mechanism involves a downregulation of ubiquitin-proteasome-mediated degradation without any change in synthetic rate." @default.
- W4249281569 created "2022-05-12" @default.
- W4249281569 creator A5014232912 @default.
- W4249281569 creator A5053350932 @default.
- W4249281569 creator A5080530083 @default.
- W4249281569 date "1999-05-18" @default.
- W4249281569 modified "2023-10-16" @default.
- W4249281569 title "Adenovirus-Mediated Overexpression of Microsomal Triglyceride Transfer Protein (MTP): Mechanistic Studies on the Role of MTP in Apolipoprotein B-100 Biogenesis" @default.
- W4249281569 cites W11746928 @default.
- W4249281569 cites W1520350378 @default.
- W4249281569 cites W1551412888 @default.
- W4249281569 cites W1591366077 @default.
- W4249281569 cites W1599366995 @default.
- W4249281569 cites W1657755666 @default.
- W4249281569 cites W1837608239 @default.
- W4249281569 cites W2002433789 @default.
- W4249281569 cites W2007208538 @default.
- W4249281569 cites W2018894673 @default.
- W4249281569 cites W2031277486 @default.
- W4249281569 cites W2060460124 @default.
- W4249281569 cites W2119472422 @default.
- W4249281569 cites W2156153564 @default.
- W4249281569 cites W2157680057 @default.
- W4249281569 cites W2182436399 @default.
- W4249281569 cites W2187723284 @default.
- W4249281569 cites W2339855910 @default.
- W4249281569 cites W249864219 @default.
- W4249281569 cites W4210953165 @default.
- W4249281569 doi "https://doi.org/10.1021/bi9904196" @default.
- W4249281569 hasPublicationYear "1999" @default.
- W4249281569 type Work @default.
- W4249281569 citedByCount "26" @default.
- W4249281569 countsByYear W42492815692012 @default.
- W4249281569 countsByYear W42492815692013 @default.
- W4249281569 countsByYear W42492815692016 @default.
- W4249281569 countsByYear W42492815692017 @default.
- W4249281569 countsByYear W42492815692018 @default.
- W4249281569 crossrefType "journal-article" @default.
- W4249281569 hasAuthorship W4249281569A5014232912 @default.
- W4249281569 hasAuthorship W4249281569A5053350932 @default.
- W4249281569 hasAuthorship W4249281569A5080530083 @default.
- W4249281569 hasConcept C104317684 @default.
- W4249281569 hasConcept C13028836 @default.
- W4249281569 hasConcept C153911025 @default.
- W4249281569 hasConcept C185592680 @default.
- W4249281569 hasConcept C2778163477 @default.
- W4249281569 hasConcept C2778913445 @default.
- W4249281569 hasConcept C2780072125 @default.
- W4249281569 hasConcept C2781403372 @default.
- W4249281569 hasConcept C54009773 @default.
- W4249281569 hasConcept C55493867 @default.
- W4249281569 hasConcept C62746215 @default.
- W4249281569 hasConcept C79879829 @default.
- W4249281569 hasConcept C8243546 @default.
- W4249281569 hasConcept C86803240 @default.
- W4249281569 hasConceptScore W4249281569C104317684 @default.
- W4249281569 hasConceptScore W4249281569C13028836 @default.
- W4249281569 hasConceptScore W4249281569C153911025 @default.
- W4249281569 hasConceptScore W4249281569C185592680 @default.
- W4249281569 hasConceptScore W4249281569C2778163477 @default.
- W4249281569 hasConceptScore W4249281569C2778913445 @default.
- W4249281569 hasConceptScore W4249281569C2780072125 @default.
- W4249281569 hasConceptScore W4249281569C2781403372 @default.
- W4249281569 hasConceptScore W4249281569C54009773 @default.
- W4249281569 hasConceptScore W4249281569C55493867 @default.
- W4249281569 hasConceptScore W4249281569C62746215 @default.
- W4249281569 hasConceptScore W4249281569C79879829 @default.
- W4249281569 hasConceptScore W4249281569C8243546 @default.
- W4249281569 hasConceptScore W4249281569C86803240 @default.
- W4249281569 hasIssue "23" @default.
- W4249281569 hasLocation W42492815691 @default.
- W4249281569 hasOpenAccess W4249281569 @default.
- W4249281569 hasPrimaryLocation W42492815691 @default.
- W4249281569 hasRelatedWork W1993099006 @default.
- W4249281569 hasRelatedWork W2006512524 @default.
- W4249281569 hasRelatedWork W2014044011 @default.
- W4249281569 hasRelatedWork W2097533439 @default.
- W4249281569 hasRelatedWork W2131553564 @default.
- W4249281569 hasRelatedWork W2137985353 @default.
- W4249281569 hasRelatedWork W2139591016 @default.
- W4249281569 hasRelatedWork W2161722711 @default.
- W4249281569 hasRelatedWork W2171313296 @default.
- W4249281569 hasRelatedWork W2398678483 @default.
- W4249281569 hasVolume "38" @default.
- W4249281569 isParatext "false" @default.
- W4249281569 isRetracted "false" @default.
- W4249281569 workType "article" @default.