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- W4249319734 abstract "This protocol describes an automated procedure for constructing an indexed Illumina DNA library. With this method, genomic DNA fragments are produced by sonication, using high-frequency acoustic energy to shear DNA. Double-stranded DNA (dsDNA) will fragment when exposed to the energy of adaptive focused acoustic shearing (AFA). The resulting DNA fragments are ligated to adaptors, amplified by polymer chain reaction (PCR), and subjected to size selection using magnetic beads. The product is suitable for use as template in whole-genome sequencing." @default.
- W4249319734 created "2022-05-12" @default.
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- W4249319734 date "2016-11-01" @default.
- W4249319734 modified "2023-10-14" @default.
- W4249319734 title "Whole-Genome Sequencing: Automated, Indexed Library Preparation" @default.
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- W4249319734 doi "https://doi.org/10.1101/pdb.prot094649" @default.
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