Matches in SemOpenAlex for { <https://semopenalex.org/work/W4249439074> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4249439074 abstract "Abstract Introduction Antibiotic resistance is a serious threat to humankind. There is need for the development of new therapeutic options. Ocimum basilicum has been used traditionally as a medicinal herb against infectious diseases and as a food additive. Objective This study aimed at evaluating the phytochemical composition and in vitro antibacterial activities of the ethyl acetate leaf extract of Ocimum basilicum . Methods Ocimum basilicum leaves were collected from Mbeere, Embu County, Kenya and ethyl acetate extraction done at Kenyatta University. Phytochemical composition was evaluated by Gas chromatograph mass spectrophotometry while antibacterial activities were evaluated by disc diffusion and broth microdilution methods. The test microorganisms were methicillin resistant S. aureus, S. aureus, P. aeruginosa and E. coli . Results The extract exhibited a broad spectrum antibacterial activity. The gram negative bacteria showed more susceptibility to the organic extract compared to the gram positive bacteria. Pseudomonas aeruginosa had the highest zone of inhibition (27.00 ± 2.00 mm), while the S. aureus isolate had the lowest zone of inhibition (24.00 ± 1.00 mm). Escherichia coli, S. aureus and MRSA species had an MIC of 62.5 mg/ml compared to P. aeruginosa with an MIC of 125mg/ml when exposed to the leaf extract. The antibacterial activity could be attributed to the synergistic effects of phytochemicals such as terpenoids, alkaloids, essential oils, fatty acids, flavonoids and aldehydes that have been known to have antimicrobial properties. A total of 30 phytochemical compounds were eluted from the extract of O. basilicum . Nootkatone, a sesquiterpenoid had the highest concentration at 20.86mg/g. The compound 1,3-Dimethyl-5-isobutylcyclohexane, a fatty acid had the lowest concentration at 0.10mg/g. Conclusion O. basilicum has antibacterial activities on the tested pathogens and can be used to treat infections. Phytochemicals with antibacterial effects in the herb can be used as lead molecules in developing new antibacterial drugs." @default.
- W4249439074 created "2022-05-12" @default.
- W4249439074 creator A5001341317 @default.
- W4249439074 creator A5025669132 @default.
- W4249439074 creator A5060313365 @default.
- W4249439074 creator A5067242241 @default.
- W4249439074 date "2020-09-28" @default.
- W4249439074 modified "2023-09-23" @default.
- W4249439074 title "Phytochemical Composition and Antibacterial Activities of the Ethyl Acetate Leaf Extract of Ocimum basilicum" @default.
- W4249439074 doi "https://doi.org/10.21203/rs.3.rs-80006/v1" @default.
- W4249439074 hasPublicationYear "2020" @default.
- W4249439074 type Work @default.
- W4249439074 citedByCount "1" @default.
- W4249439074 countsByYear W42494390742023 @default.
- W4249439074 crossrefType "posted-content" @default.
- W4249439074 hasAuthorship W4249439074A5001341317 @default.
- W4249439074 hasAuthorship W4249439074A5025669132 @default.
- W4249439074 hasAuthorship W4249439074A5060313365 @default.
- W4249439074 hasAuthorship W4249439074A5067242241 @default.
- W4249439074 hasBestOaLocation W42494390741 @default.
- W4249439074 hasConcept C11636898 @default.
- W4249439074 hasConcept C176947019 @default.
- W4249439074 hasConcept C185592680 @default.
- W4249439074 hasConcept C195475562 @default.
- W4249439074 hasConcept C206103511 @default.
- W4249439074 hasConcept C2777612184 @default.
- W4249439074 hasConcept C2779342943 @default.
- W4249439074 hasConcept C2779858419 @default.
- W4249439074 hasConcept C2780104969 @default.
- W4249439074 hasConcept C2780950330 @default.
- W4249439074 hasConcept C2910666622 @default.
- W4249439074 hasConcept C31903555 @default.
- W4249439074 hasConcept C43617362 @default.
- W4249439074 hasConcept C4937899 @default.
- W4249439074 hasConcept C523546767 @default.
- W4249439074 hasConcept C54355233 @default.
- W4249439074 hasConcept C55493867 @default.
- W4249439074 hasConcept C556039675 @default.
- W4249439074 hasConcept C71924100 @default.
- W4249439074 hasConcept C86803240 @default.
- W4249439074 hasConcept C89423630 @default.
- W4249439074 hasConceptScore W4249439074C11636898 @default.
- W4249439074 hasConceptScore W4249439074C176947019 @default.
- W4249439074 hasConceptScore W4249439074C185592680 @default.
- W4249439074 hasConceptScore W4249439074C195475562 @default.
- W4249439074 hasConceptScore W4249439074C206103511 @default.
- W4249439074 hasConceptScore W4249439074C2777612184 @default.
- W4249439074 hasConceptScore W4249439074C2779342943 @default.
- W4249439074 hasConceptScore W4249439074C2779858419 @default.
- W4249439074 hasConceptScore W4249439074C2780104969 @default.
- W4249439074 hasConceptScore W4249439074C2780950330 @default.
- W4249439074 hasConceptScore W4249439074C2910666622 @default.
- W4249439074 hasConceptScore W4249439074C31903555 @default.
- W4249439074 hasConceptScore W4249439074C43617362 @default.
- W4249439074 hasConceptScore W4249439074C4937899 @default.
- W4249439074 hasConceptScore W4249439074C523546767 @default.
- W4249439074 hasConceptScore W4249439074C54355233 @default.
- W4249439074 hasConceptScore W4249439074C55493867 @default.
- W4249439074 hasConceptScore W4249439074C556039675 @default.
- W4249439074 hasConceptScore W4249439074C71924100 @default.
- W4249439074 hasConceptScore W4249439074C86803240 @default.
- W4249439074 hasConceptScore W4249439074C89423630 @default.
- W4249439074 hasLocation W42494390741 @default.
- W4249439074 hasOpenAccess W4249439074 @default.
- W4249439074 hasPrimaryLocation W42494390741 @default.
- W4249439074 hasRelatedWork W1593108952 @default.
- W4249439074 hasRelatedWork W2058493762 @default.
- W4249439074 hasRelatedWork W2557816911 @default.
- W4249439074 hasRelatedWork W3010352256 @default.
- W4249439074 hasRelatedWork W4210540048 @default.
- W4249439074 hasRelatedWork W4249439074 @default.
- W4249439074 hasRelatedWork W4256180810 @default.
- W4249439074 hasRelatedWork W4312803370 @default.
- W4249439074 hasRelatedWork W2186889101 @default.
- W4249439074 hasRelatedWork W2330796871 @default.
- W4249439074 isParatext "false" @default.
- W4249439074 isRetracted "false" @default.
- W4249439074 workType "article" @default.