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• W4249441119 abstract "In plant cells nucleolar fibrillar centres (FCs) undergo ultrastructural changes, depending on the nucleolar activity. We have found two types of FC structure in nucleoli with either high or low activity, to which we have given the conventional names of homogeneous and heterogeneous, respectively. The first type is characterized by the presence of fibres that we describe structurally and cytochemically as decondensed chromatin; the second type, in addition to these fibres, contains a variable number of dense cores made up of condensed chromatin. Moreover, RNA does not appear to be present in FCs, while proteins are a major component. Autoradiography after tritiated uridine incorporation shows that FCs are not the site of transcription, but that this takes place in the fibrillar component; the same result is obtained using the lead acetate fixation technique for detecting orthophosphate ions. This fact leads us to think that FCs are not the whole interphasic counterpart of the mitotic nucleolar organizing region (NOR), as stated by other authors, but only the portion of the NOR that is temporarily inactive in transcription; the transcriptionally active part of the NOR is in the fibrillar component, bound to its earliest product of transcription. Thus, FCs and the fibrillar component constitute a functional unit." @default.
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• W4249441119 date "1982-12-01" @default.
• W4249441119 modified "2023-09-25" @default.
• W4249441119 title "Nucleolar fibrillar centres in plant meristematic cells: Ultrastructure, cytochemistry and autoradiography*" @default.
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• W4249441119 doi "https://doi.org/10.1242/jcs.58.1.313" @default.
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