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- W4249583561 abstract "(Cell 154, 391–402; July 18, 2013) In the article above, we documented coordinated movements between the mitotic spindle and the cell cortex that resulted in centering of the spindle during mitosis in HeLa cells. To do this, we conducted time-lapse imaging of mCherry-H2B- and Lifeact-mCherry-expressing HeLa cells undergoing mitosis. The data from these time-lapse experiments were presented in multiple ways: as supplemental movies, kymographs, still frames, and overlaid images that allowed us to clearly visualize the changes in membrane position between early and late time points during anaphase. To enable a direct comparison between these different presentation methods, the same time-lapse sequences are visualized using each method in the paper. However, for Figures 5A, 5G, and 6D, we neglected to explicitly state in the figure legends the ways in which the same images are displayed in different places as part of this analysis. To avoid any confusion and in accordance with the Cell Press policy regarding clarity in data presentation, below we make clear all of these instances. The type I control cell is presented in Figures 4D, 4E, and 5A and Movie S1. The type I LGN+4.1 RNAi cell is presented in Figures 4D and 4E and Movie S2. The type III control cell is presented in Figures 5A, 5B, and 5C and Movie S4. The type II control cell is presented in Figures 5B and 5C and Movie S5. The type III LGN+ 4.1 RNAi is presented in Figures 5D and 5G and Movie S6. Finally, we conducted time-lapse imaging of GFP-Anillin localization during anaphase. A time-lapse sequence visualizing GFP-Anillin is shown in Figure 6B and Movie S7. We also analyzed these images to quantify the relative cortical fluorescence around the cell, and an image from this sequence is reproduced in Figure 6D to illustrate how the fluorescence quantification was conducted and to provide comparison to GFP-MRLC localization. Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in AnaphaseKiyomitsu et al.CellJuly 18, 2013In BriefTo ensure symmetric division, cells adjust the centering of the mitotic spindle during anaphase by two mechanisms, one of which involves asymmetric plasma membrane elongation away from the uncentered chromosomes to reset the relative spindle positioning to a newly created cell center. Full-Text PDF Open Archive" @default.
- W4249583561 created "2022-05-12" @default.
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- W4249583561 date "2013-09-01" @default.
- W4249583561 modified "2023-10-16" @default.
- W4249583561 title "Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase" @default.
- W4249583561 doi "https://doi.org/10.1016/j.cell.2013.08.036" @default.
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