Matches in SemOpenAlex for { <https://semopenalex.org/work/W4249737296> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W4249737296 endingPage "4686" @default.
- W4249737296 startingPage "4679" @default.
- W4249737296 abstract "We used a new approach called panhandle polymerase chain reaction (PCR) to clone an MLL genomic translocation breakpoint in a case of acute lymphoblastic leukemia of infancy in which karyotype analysis was technically unsuccessful and did not show the translocation partner. Panhandle PCR amplified known MLL sequence 5′ of the breakpoint and 3′ sequence from the unknown partner gene from a DNA template with an intrastrand loop schematically shaped like a pan with a handle. The 7-kb panhandle PCR product contained the translocation breakpoint in MLL intron 8. The partner DNA included unique nonrepetitive sequences, Alu and mammalian apparent LTR-retrotransposon (MaLR) repetitive sequences, and a region of homology to expressed sequence tags. MaLR sequences have not been found before near leukemia-associated translocation breakpoints. The nonrepetitive sequences were not homologous to known partner genes of MLL. Screening of somatic cell hybrid and radiation hybrid lines by PCR and fluorescence in situ hybridization analysis of normal metaphase chromosomes mapped the partner DNA to chromosome band 4q21. Reverse transcriptase-PCR identified an MLL-AF-4 chimeric mRNA, indicating that panhandle PCR identified a fusion of MLL with a previously uncharacterized AF-4 intronic sequence. Panhandle PCR facilitates cloning translocation breakpoints and identifying unknown partner genes." @default.
- W4249737296 created "2022-05-12" @default.
- W4249737296 creator A5002807620 @default.
- W4249737296 creator A5018786888 @default.
- W4249737296 creator A5028020710 @default.
- W4249737296 creator A5030535866 @default.
- W4249737296 creator A5042587684 @default.
- W4249737296 creator A5043048844 @default.
- W4249737296 creator A5055052725 @default.
- W4249737296 creator A5063783317 @default.
- W4249737296 creator A5079843979 @default.
- W4249737296 creator A5086288252 @default.
- W4249737296 creator A5090139384 @default.
- W4249737296 date "1997-12-15" @default.
- W4249737296 modified "2023-09-28" @default.
- W4249737296 title "Panhandle Polymerase Chain Reaction Amplifies MLL Genomic Translocation Breakpoint Involving Unknown Partner Gene" @default.
- W4249737296 cites W1483545822 @default.
- W4249737296 cites W1594965066 @default.
- W4249737296 cites W1982640053 @default.
- W4249737296 cites W1990863885 @default.
- W4249737296 cites W2036296056 @default.
- W4249737296 cites W2040890104 @default.
- W4249737296 cites W2048492425 @default.
- W4249737296 cites W2065130184 @default.
- W4249737296 cites W2128750289 @default.
- W4249737296 cites W2130924644 @default.
- W4249737296 cites W2149857208 @default.
- W4249737296 cites W2164796388 @default.
- W4249737296 cites W2255761522 @default.
- W4249737296 cites W2262476462 @default.
- W4249737296 cites W2339378175 @default.
- W4249737296 cites W237111889 @default.
- W4249737296 cites W2407641423 @default.
- W4249737296 cites W264856516 @default.
- W4249737296 cites W4230006048 @default.
- W4249737296 cites W4242999238 @default.
- W4249737296 cites W69594174 @default.
- W4249737296 doi "https://doi.org/10.1182/blood.v90.12.4679.4679_4679_4686" @default.
- W4249737296 hasPublicationYear "1997" @default.
- W4249737296 type Work @default.
- W4249737296 citedByCount "3" @default.
- W4249737296 crossrefType "journal-article" @default.
- W4249737296 hasAuthorship W4249737296A5002807620 @default.
- W4249737296 hasAuthorship W4249737296A5018786888 @default.
- W4249737296 hasAuthorship W4249737296A5028020710 @default.
- W4249737296 hasAuthorship W4249737296A5030535866 @default.
- W4249737296 hasAuthorship W4249737296A5042587684 @default.
- W4249737296 hasAuthorship W4249737296A5043048844 @default.
- W4249737296 hasAuthorship W4249737296A5055052725 @default.
- W4249737296 hasAuthorship W4249737296A5063783317 @default.
- W4249737296 hasAuthorship W4249737296A5079843979 @default.
- W4249737296 hasAuthorship W4249737296A5086288252 @default.
- W4249737296 hasAuthorship W4249737296A5090139384 @default.
- W4249737296 hasBestOaLocation W42497372961 @default.
- W4249737296 hasConcept C104317684 @default.
- W4249737296 hasConcept C138626823 @default.
- W4249737296 hasConcept C153911025 @default.
- W4249737296 hasConcept C20682886 @default.
- W4249737296 hasConcept C206936463 @default.
- W4249737296 hasConcept C49105822 @default.
- W4249737296 hasConcept C54355233 @default.
- W4249737296 hasConcept C86803240 @default.
- W4249737296 hasConcept C90583042 @default.
- W4249737296 hasConceptScore W4249737296C104317684 @default.
- W4249737296 hasConceptScore W4249737296C138626823 @default.
- W4249737296 hasConceptScore W4249737296C153911025 @default.
- W4249737296 hasConceptScore W4249737296C20682886 @default.
- W4249737296 hasConceptScore W4249737296C206936463 @default.
- W4249737296 hasConceptScore W4249737296C49105822 @default.
- W4249737296 hasConceptScore W4249737296C54355233 @default.
- W4249737296 hasConceptScore W4249737296C86803240 @default.
- W4249737296 hasConceptScore W4249737296C90583042 @default.
- W4249737296 hasIssue "12" @default.
- W4249737296 hasLocation W42497372961 @default.
- W4249737296 hasOpenAccess W4249737296 @default.
- W4249737296 hasPrimaryLocation W42497372961 @default.
- W4249737296 hasRelatedWork W16950770 @default.
- W4249737296 hasRelatedWork W18994220 @default.
- W4249737296 hasRelatedWork W21787055 @default.
- W4249737296 hasRelatedWork W31386547 @default.
- W4249737296 hasRelatedWork W36484103 @default.
- W4249737296 hasRelatedWork W39392360 @default.
- W4249737296 hasRelatedWork W40774725 @default.
- W4249737296 hasRelatedWork W43838109 @default.
- W4249737296 hasRelatedWork W46146609 @default.
- W4249737296 hasRelatedWork W5268884 @default.
- W4249737296 hasVolume "90" @default.
- W4249737296 isParatext "false" @default.
- W4249737296 isRetracted "false" @default.
- W4249737296 workType "article" @default.