FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4249833567> ?p ?o ?g. }

• W4249833567 abstract "Abstract. Phosphorus (P) is an essential nutrient for living organisms. Under P-limiting conditions plants and microorganisms can exude extracellular phosphatases that release inorganic phosphate (Pi) from organic phosphorus compounds (Porg). Phytic acid (IP6) is an important form of Porg in many soils. The enzymatic hydrolysis of IP6 by phytase yields plant available inorganic phosphate (Pi) and less phosphorylated inositol derivates as products. The hydrolysis of organic P-compounds by phosphatases leaves an isotopic imprint on the oxygen isotope composition (δ18O) of released Pi, which might be used to trace P in the environment. This study aims at determining the effect of phytase on the oxygen isotope composition of released Pi. For this purpose, enzymatic assays with histidine acid phytases from wheat and Aspergillus niger were prepared using IP6, adenosine 5'monophosphate (AMP) and glycerophosphate (GPO4) as substrates. For a comparison to the δ18O of Pi released by other extracellular enzymes, enzymatic assays with acid phosphatases from potato and wheat germ with IP6 as substrate were prepared. During the hydrolysis of IP6 by phytase, four Pi are released, and one oxygen atom from water is incorporated into each Pi. This incorporation of oxygen from water into Pi is subject to an apparent inverse isotopic fractionation (&amp;varepsilon; ∼ 6 to 10‰), which is similar to that imparted by acid phosphatase from potato during the hydrolysis of IP6 (&amp;varepsilon; ∼ 7‰) where less than three Pi are released. The incorporation of oxygen from water into Pi during the hydrolysis of AMP and GPO4 by phytase yielded a normal isotopic fractionation (&amp;varepsilon; ∼ −12‰), again similar to values reported for acid phosphatases from potato and wheat germ. We attribute this similarity in ε to the same amino acid sequence motif (RHGXRXP) at the active site of these enzymes, which leads to similar reaction mechanisms. We suggest that the striking substrate-dependency of the isotopic fractionation could be attributed to a difference in the δ18O-values of the C-O-P bridging and non-bridging oxygen atoms in organic phosphate compounds." @default.
• W4249833567 created "2022-05-12" @default.
• W4249833567 creator A5018500949 @default.
• W4249833567 creator A5019388262 @default.
• W4249833567 creator A5066103423 @default.
• W4249833567 creator A5081994751 @default.
• W4249833567 creator A5083167052 @default.
• W4249833567 date "2015-03-30" @default.
• W4249833567 modified "2023-09-27" @default.
• W4249833567 title "The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and <i>Aspergillus niger</i> phytase" @default.
• W4249833567 cites W118500023 @default.
• W4249833567 cites W1820831325 @default.
• W4249833567 cites W1964806509 @default.
• W4249833567 cites W1966145007 @default.
• W4249833567 cites W1972975493 @default.
• W4249833567 cites W1992532108 @default.
• W4249833567 cites W1996832202 @default.
• W4249833567 cites W1999921589 @default.
• W4249833567 cites W2014214752 @default.
• W4249833567 cites W2019197671 @default.
• W4249833567 cites W2025543495 @default.
• W4249833567 cites W2034516828 @default.
• W4249833567 cites W2053489320 @default.
• W4249833567 cites W2063401196 @default.
• W4249833567 cites W2074046740 @default.
• W4249833567 cites W2078789085 @default.
• W4249833567 cites W2082076816 @default.
• W4249833567 cites W2090180555 @default.
• W4249833567 cites W2092436758 @default.
• W4249833567 cites W2114437147 @default.
• W4249833567 cites W2123863099 @default.
• W4249833567 cites W2141542167 @default.
• W4249833567 cites W2163763262 @default.
• W4249833567 cites W2165112476 @default.
• W4249833567 cites W2167750324 @default.
• W4249833567 cites W2315135395 @default.
• W4249833567 cites W2317970066 @default.
• W4249833567 cites W2323915646 @default.
• W4249833567 cites W2326727094 @default.
• W4249833567 cites W4211083240 @default.
• W4249833567 doi "https://doi.org/10.5194/bgd-12-5055-2015" @default.
• W4249833567 hasPublicationYear "2015" @default.
• W4249833567 type Work @default.
• W4249833567 citedByCount "1" @default.
• W4249833567 countsByYear W42498335672022 @default.
• W4249833567 crossrefType "posted-content" @default.
• W4249833567 hasAuthorship W4249833567A5018500949 @default.
• W4249833567 hasAuthorship W4249833567A5019388262 @default.
• W4249833567 hasAuthorship W4249833567A5066103423 @default.
• W4249833567 hasAuthorship W4249833567A5081994751 @default.
• W4249833567 hasAuthorship W4249833567A5083167052 @default.
• W4249833567 hasBestOaLocation W42498335671 @default.
• W4249833567 hasConcept C109551439 @default.
• W4249833567 hasConcept C178666793 @default.
• W4249833567 hasConcept C178790620 @default.
• W4249833567 hasConcept C181199279 @default.
• W4249833567 hasConcept C185592680 @default.
• W4249833567 hasConcept C2775960407 @default.
• W4249833567 hasConcept C2777132085 @default.
• W4249833567 hasConcept C2778036281 @default.
• W4249833567 hasConcept C510538283 @default.
• W4249833567 hasConcept C55493867 @default.
• W4249833567 hasConcept C94412978 @default.
• W4249833567 hasConceptScore W4249833567C109551439 @default.
• W4249833567 hasConceptScore W4249833567C178666793 @default.
• W4249833567 hasConceptScore W4249833567C178790620 @default.
• W4249833567 hasConceptScore W4249833567C181199279 @default.
• W4249833567 hasConceptScore W4249833567C185592680 @default.
• W4249833567 hasConceptScore W4249833567C2775960407 @default.
• W4249833567 hasConceptScore W4249833567C2777132085 @default.
• W4249833567 hasConceptScore W4249833567C2778036281 @default.
• W4249833567 hasConceptScore W4249833567C510538283 @default.
• W4249833567 hasConceptScore W4249833567C55493867 @default.
• W4249833567 hasConceptScore W4249833567C94412978 @default.
• W4249833567 hasLocation W42498335671 @default.
• W4249833567 hasLocation W42498335672 @default.
• W4249833567 hasOpenAccess W4249833567 @default.
• W4249833567 hasPrimaryLocation W42498335671 @default.
• W4249833567 hasRelatedWork W19949609 @default.
• W4249833567 hasRelatedWork W21376089 @default.
• W4249833567 hasRelatedWork W24055242 @default.
• W4249833567 hasRelatedWork W28247693 @default.
• W4249833567 hasRelatedWork W32984611 @default.
• W4249833567 hasRelatedWork W44812656 @default.
• W4249833567 hasRelatedWork W45181366 @default.
• W4249833567 hasRelatedWork W590114 @default.
• W4249833567 hasRelatedWork W931622 @default.
• W4249833567 hasRelatedWork W9871381 @default.
• W4249833567 isParatext "false" @default.
• W4249833567 isRetracted "false" @default.
• W4249833567 workType "article" @default.