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- W4249846399 abstract "Abstract Background: The biomanufacturing of D-glucaric acid has been attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for D-glucaric acid production. Results: The opi1 gene was knocked out because of its negative regulation on myo -inositol synthesis, which is the limiting step of D-glucaric acid production by S. cerevisiae . Then we constructed the biosynthetic pathway of D-glucaric acid in S. cerevisiae INVSc1 opi1Δ and obtained two engineered strains, LGA-1 and LGA-C, producing record breaking titers of D-glucaric acid, 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L D-glucaric acid from 30 g/L glucose and 10.8 g/L myo -inositol in the mode of fed-batch fermentation, respectively. However, LGA-1 was more preferable because of the genetic stability and the outperformance in applications. So far, there have been no reports on D-glucaric acid production from lignocellulose. Therefore, the biorefinery processes including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated and compared. CBP by an artificial microbial consortium composed of Trichoderma reesei Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high D-glucaric acid titers and yields after 7 d fermentation, 0.54 ± 0.12 g/L D-glucaric acid from 15 g/L Avicel, and 0.45 ± 0.06 g/L D-glucaric acid from 15 g/L steam exploded corn stover (SECS), respectively. In attempts to design the microbial consortium for more efficient CBP the team consisted of the two members, T. reesei Rut-C30 and S. cerevisiae LGA-1, was found to be the best with excellent work distribution and collaboration.Conclusions: Two engineered S. cerevisiae strains, LGA-1 and LGA-C, with high titers of D-glucaric acid were obtained. This indicates that S. cerevisiae INVSc1 was an excellent host. Lignocellulose is a more preferable substrate than myo -inositol. SHF, SSF and CBP were studied and CBP by an artificial microbial consortium of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be promising because of the relatively high titer and yield. T. reesei Rut-C30 and S. cerevisiae LGA-1were proved to be the best teammates for CBP. Further work should be done to improve the efficiency of this microbial consortium for D-glucaric acid production from lignocellulose." @default.
- W4249846399 created "2022-05-12" @default.
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- W4249846399 date "2021-01-28" @default.
- W4249846399 modified "2023-09-23" @default.
- W4249846399 title "Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium" @default.
- W4249846399 doi "https://doi.org/10.21203/rs.3.rs-29885/v2" @default.
- W4249846399 hasPublicationYear "2021" @default.
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