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- W4250083221 abstract "Normal Chinese hamster cells contain two copies of the uridine 5'-phosphate (UMP) synthase gene, which maps on hamster chromosome 4p2. By alternating the selective drug pressure, it is possible to go through multiple cycles of amplification and deamplification in a single cell lineage. To determine whether deamplification was a spontaneous event in the amplified cell population, the UMP synthase activity of the cells was checked following growth of the cells in the absence of selective pressure. Initial amplification produced a cell line in which UMP synthase is stably amplified as a homogeneously staining region (HSR) on M3. Amplification and deamplification in this system are accompanied by formation and deletion of expanded chromosomal regions carrying the amplified copies of the UMP synthase genes. Cells selected in the initial amplification step developed an HSR as determined by G-banding." @default.
- W4250083221 created "2022-05-12" @default.
- W4250083221 creator A5039855573 @default.
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- W4250083221 date "2020-08-26" @default.
- W4250083221 modified "2023-10-14" @default.
- W4250083221 title "A Reversible Selection System for UMP Synthase Gene Amplification and Deamplification" @default.
- W4250083221 doi "https://doi.org/10.1201/9781003066989-23" @default.
- W4250083221 hasPublicationYear "2020" @default.
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