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- W4250126092 abstract "Abstract This unit presents procedures for manipulating solutions of single‐ or double‐ stranded DNA through purification and concentration steps. These techniques are useful when proteins or solute molecules need to be removed from aqueous solutions, or when DNA solutions need to be concentrated. The basic protocol, using phenol extraction and ethanol (or isopropanol) precipitation, is appropriate for the purification of DNA from small volumes (<0.4 ml) at concentrations lower than 1 mg/ml. Three support protocols outline methods to buffer the phenol used in extractions, concentrate DNA using butanol, and extract residual organic solvents with ether. An alternative to these methods is nucleic acid purification using glass beads and this is also presented. These protocols may also be used for purifying RNA. Two alternate protocols can be used for concentrating RNA and extracting and precipitating DNA from larger volumes and from dilute solutions, and for removing low molecular weight oligonucleotides and triphosphates." @default.
- W4250126092 created "2022-05-12" @default.
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- W4250126092 date "1998-03-01" @default.
- W4250126092 modified "2023-09-27" @default.
- W4250126092 title "Purification and Concentration of DNA from Aqueous Solutions" @default.
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- W4250126092 doi "https://doi.org/10.1002/0471141755.pha03cs00" @default.
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