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- W4250273228 abstract "<h3>Gene Construction and Modification</h3> As our understanding of genetics and molecular biology deepens, some central concepts have required modification. One of these, the doctrine of one gene—one polypeptide, must now be altered, at least from its simplest form. Hozumi and Tonegawa<sup>1</sup>have found that the gene loci for the constant and variable regions of the<i>k</i>light chain of the Ig molecule are separated in the genome (chromosome) of the embryo but are adjacent in the genome of the adult animal. The experiment first required that the DNA be fragmented by restriction enzyme digestion and the fragments separated according to molecular size by preparative agarose gel electrophoresis. The location of the variable and constant regions was achieved by hybridizing with iodine 125—labeled messenger RNA, prepared from a myeloma tissue culture line (MOPC 321) secreting the relevant light chain. The pattern of labeling obtained with embryonic DNA indicated that the" @default.
- W4250273228 created "2022-05-12" @default.
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- W4250273228 date "1980-06-06" @default.
- W4250273228 modified "2023-09-26" @default.
- W4250273228 title "Genetics" @default.
- W4250273228 doi "https://doi.org/10.1001/jama.1980.03300470046027" @default.
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