Matches in SemOpenAlex for { <https://semopenalex.org/work/W4250511458> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4250511458 abstract "The crucial dependence of normal automaticity of sinoatrial nodal cells (SANC) on CaMKII signaling has previously been linked to an effect to facilitate recovery from inactivation of L-type Ca 2+ channels. More recently, however, it has been discovered that spontaneous, rhythmic, local Ca 2+ releases (LCR’s) from sarcoplasmic reticulum (SR) activate Na + /Ca 2+ exchanger current, imparting an exponential increase to the later part of the spontaneous depolarization that brings the surface membrane to threshold to fire an action potential (AP). Here, in single isolated intact SANC, using the phosphorylation site-specific polyclonal antibody, we show (Fig. A ) that, basal state phospholamban (PLB) phosphorylation at Thr-17, a CaMKII phosphorylation site, is over 3 times greater in SANC than in ventricular cells (VC). The CaMKII inhibitor, KN-93, but not its inactive analog, KN-92, markedly inhibits (by 80%) PLB Thr-17 phosphorylation (Fig. B ). Confocal imaging of saponin permeablized SANC, superfused in physiological solution with 150nM free Ca 2+ and 0.5mM EGTA, showed that KN-93 reduced the LCR frequency by 80% (from 5.6 ± 1.5 to 1.1 ± 0.3/1s × 100 μm) and size by 50% (from 4.0 ± 0.3 to 2.0 ± 0.3 μm). Thus, in addition to an effect on L-type Ca 2+ channels, the high basal CaMKII activation (indexed by PLB phosphorylation at Thr-17) has a major effect to determine the characteristics of spontaneous LCR’s that initiate AP’s and control normal automaticity of SANC." @default.
- W4250511458 created "2022-05-12" @default.
- W4250511458 creator A5010362119 @default.
- W4250511458 creator A5039739686 @default.
- W4250511458 creator A5044367029 @default.
- W4250511458 creator A5045563275 @default.
- W4250511458 creator A5048711709 @default.
- W4250511458 creator A5061406584 @default.
- W4250511458 creator A5075584064 @default.
- W4250511458 creator A5081151780 @default.
- W4250511458 creator A5091904163 @default.
- W4250511458 date "2007-10-16" @default.
- W4250511458 modified "2023-09-25" @default.
- W4250511458 title "Abstract 500: High Basal Ca <sup>2+</sup> / Calmodulin Kinase II Activity Modulates Spontaneous Sarcoplasmic Reticulum Ca <sup>2+</sup> Cycling That Drives Normal Automaticity in Sinoatrial Nodal Cells" @default.
- W4250511458 doi "https://doi.org/10.1161/circ.116.suppl_16.ii_86-c" @default.
- W4250511458 hasPublicationYear "2007" @default.
- W4250511458 type Work @default.
- W4250511458 citedByCount "1" @default.
- W4250511458 crossrefType "journal-article" @default.
- W4250511458 hasAuthorship W4250511458A5010362119 @default.
- W4250511458 hasAuthorship W4250511458A5039739686 @default.
- W4250511458 hasAuthorship W4250511458A5044367029 @default.
- W4250511458 hasAuthorship W4250511458A5045563275 @default.
- W4250511458 hasAuthorship W4250511458A5048711709 @default.
- W4250511458 hasAuthorship W4250511458A5061406584 @default.
- W4250511458 hasAuthorship W4250511458A5075584064 @default.
- W4250511458 hasAuthorship W4250511458A5081151780 @default.
- W4250511458 hasAuthorship W4250511458A5091904163 @default.
- W4250511458 hasConcept C113217602 @default.
- W4250511458 hasConcept C11960822 @default.
- W4250511458 hasConcept C12554922 @default.
- W4250511458 hasConcept C126322002 @default.
- W4250511458 hasConcept C134018914 @default.
- W4250511458 hasConcept C158617107 @default.
- W4250511458 hasConcept C17137333 @default.
- W4250511458 hasConcept C185592680 @default.
- W4250511458 hasConcept C2776989580 @default.
- W4250511458 hasConcept C2777953023 @default.
- W4250511458 hasConcept C2778542873 @default.
- W4250511458 hasConcept C4141045 @default.
- W4250511458 hasConcept C519063684 @default.
- W4250511458 hasConcept C71924100 @default.
- W4250511458 hasConcept C84393581 @default.
- W4250511458 hasConcept C86803240 @default.
- W4250511458 hasConcept C95444343 @default.
- W4250511458 hasConceptScore W4250511458C113217602 @default.
- W4250511458 hasConceptScore W4250511458C11960822 @default.
- W4250511458 hasConceptScore W4250511458C12554922 @default.
- W4250511458 hasConceptScore W4250511458C126322002 @default.
- W4250511458 hasConceptScore W4250511458C134018914 @default.
- W4250511458 hasConceptScore W4250511458C158617107 @default.
- W4250511458 hasConceptScore W4250511458C17137333 @default.
- W4250511458 hasConceptScore W4250511458C185592680 @default.
- W4250511458 hasConceptScore W4250511458C2776989580 @default.
- W4250511458 hasConceptScore W4250511458C2777953023 @default.
- W4250511458 hasConceptScore W4250511458C2778542873 @default.
- W4250511458 hasConceptScore W4250511458C4141045 @default.
- W4250511458 hasConceptScore W4250511458C519063684 @default.
- W4250511458 hasConceptScore W4250511458C71924100 @default.
- W4250511458 hasConceptScore W4250511458C84393581 @default.
- W4250511458 hasConceptScore W4250511458C86803240 @default.
- W4250511458 hasConceptScore W4250511458C95444343 @default.
- W4250511458 hasIssue "suppl_16" @default.
- W4250511458 hasLocation W42505114581 @default.
- W4250511458 hasOpenAccess W4250511458 @default.
- W4250511458 hasPrimaryLocation W42505114581 @default.
- W4250511458 hasRelatedWork W1964461691 @default.
- W4250511458 hasRelatedWork W1977626206 @default.
- W4250511458 hasRelatedWork W2066118117 @default.
- W4250511458 hasRelatedWork W2093507972 @default.
- W4250511458 hasRelatedWork W2093562584 @default.
- W4250511458 hasRelatedWork W2169854908 @default.
- W4250511458 hasRelatedWork W2240881721 @default.
- W4250511458 hasRelatedWork W2399001178 @default.
- W4250511458 hasRelatedWork W2461290894 @default.
- W4250511458 hasRelatedWork W995184095 @default.
- W4250511458 hasVolume "116" @default.
- W4250511458 isParatext "false" @default.
- W4250511458 isRetracted "false" @default.
- W4250511458 workType "article" @default.