FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4250769951> ?p ?o ?g. }

• W4250769951 endingPage "F825" @default.
• W4250769951 startingPage "F819" @default.
• W4250769951 abstract "First published August 9, 2001; 10.1152/ajprenal.0075.2001.—We have reported that nitric oxide (NO) inhibits thick ascending limb (THAL) chloride absorption ( J Cl − ). NaCl transport in the THAL depends on apical Na + -K + -2Cl − cotransporters, apical K + channels, and basolateral Na + -K + -ATPase. However, the transporter inhibited by NO is unknown. We hypothesized that NO decreases THAL J Cl − by inhibiting the Na + -K + -2Cl − cotransporter. THALs from Sprague-Dawley rats were isolated and perfused. Intracellular sodium ([Na + ] i ) and chloride concentrations ([Cl − ] i ) were measured with sodium green and SPQ, respectively. The NO donor spermine NONOate (SPM) decreased [Na + ] i from 13.5 ± 1.2 to 9.6 ± 1.6 mM ( P < 0.05) and also decreased [Cl − ] i ( P < 0.01). We next tested whether NO decreases Na + -K + -2Cl − cotransporter activity by measuring the initial rate of Na + transport. In the presence of SPM in the bath, initial rates of Na + entry were 49.6 ± 6.0% slower compared with control rates ( P < 0.05). To determine whether NO inhibits apical K + channel activity, we measured the change in membrane potential caused by an increase in luminal K + from 1 to 25 mM using a potential-sensitive fluorescent dye. In the presence of SPM, increasing luminal K + concentration depolarized THALs to the same extent as it did in control tubules. We then tested whether a change in apical K + permeability could affect NO-induced inhibition of THAL J Cl − . In the presence of luminal valinomycin, which increases K + permeability, addition of SPM decreased THAL J Cl − by 41.2 ± 10.4%, not significantly different from the inhibition observed in control tubules. We finally tested whether NO alters the affinity or maximal rate of Na + -K + -ATPase by measuring oxygen consumption rate (Qo 2 ) in THAL suspensions in the presence of nystatin in varying concentrations of Na + . In the presence of 10.5 mM Na + , nystatin increased Qo 2 to 119.1 ± 19.2 and 125.6 ± 23.4 nmol O 2 · mg protein −1 · min −1 in SPM- and furosemide-treated tubules, respectively. In the presence of 145 mM extracellular Na + , nystatin increased Qo 2 by 104 ± 7 and 94 ± 20% in NO- and furosemide-treated tubules, respectively. We concluded that NO decreases THAL J Cl − by inhibiting Na + -K + -2Cl − cotransport rather than inhibiting apical K + channels or the sodium pump." @default.
• W4250769951 created "2022-05-12" @default.
• W4250769951 creator A5021025572 @default.
• W4250769951 creator A5072291294 @default.
• W4250769951 creator A5077979393 @default.
• W4250769951 date "2001-11-01" @default.
• W4250769951 modified "2023-10-15" @default.
• W4250769951 title "NO decreases thick ascending limb chloride absorption by reducing Na⁺-K⁺-2Cl⁻cotransporter activity" @default.
• W4250769951 cites W1552509407 @default.
• W4250769951 cites W1585150602 @default.
• W4250769951 cites W1854895726 @default.
• W4250769951 cites W1898513650 @default.
• W4250769951 cites W1925196084 @default.
• W4250769951 cites W1964033398 @default.
• W4250769951 cites W1966895188 @default.
• W4250769951 cites W1983102581 @default.
• W4250769951 cites W1990665368 @default.
• W4250769951 cites W2032890845 @default.
• W4250769951 cites W2033595361 @default.
• W4250769951 cites W2044108046 @default.
• W4250769951 cites W2047545717 @default.
• W4250769951 cites W2050216269 @default.
• W4250769951 cites W2052225522 @default.
• W4250769951 cites W2076961471 @default.
• W4250769951 cites W2080942008 @default.
• W4250769951 cites W2098009074 @default.
• W4250769951 cites W2119375870 @default.
• W4250769951 cites W2124942654 @default.
• W4250769951 cites W2158738489 @default.
• W4250769951 cites W2161834399 @default.
• W4250769951 cites W2162293702 @default.
• W4250769951 cites W2183071466 @default.
• W4250769951 cites W2183602311 @default.
• W4250769951 cites W2185146306 @default.
• W4250769951 cites W2188551092 @default.
• W4250769951 cites W2282258393 @default.
• W4250769951 cites W2283921235 @default.
• W4250769951 cites W2319841016 @default.
• W4250769951 cites W2343158484 @default.
• W4250769951 cites W2400187764 @default.
• W4250769951 cites W2411219465 @default.
• W4250769951 cites W2412915627 @default.
• W4250769951 doi "https://doi.org/10.1152/ajprenal.0075.2001" @default.
• W4250769951 hasPublicationYear "2001" @default.
• W4250769951 type Work @default.
• W4250769951 citedByCount "104" @default.
• W4250769951 countsByYear W42507699512012 @default.
• W4250769951 countsByYear W42507699512013 @default.
• W4250769951 countsByYear W42507699512014 @default.
• W4250769951 countsByYear W42507699512015 @default.
• W4250769951 countsByYear W42507699512016 @default.
• W4250769951 countsByYear W42507699512017 @default.
• W4250769951 countsByYear W42507699512018 @default.
• W4250769951 countsByYear W42507699512019 @default.
• W4250769951 countsByYear W42507699512020 @default.
• W4250769951 crossrefType "journal-article" @default.
• W4250769951 hasAuthorship W4250769951A5021025572 @default.
• W4250769951 hasAuthorship W4250769951A5072291294 @default.
• W4250769951 hasAuthorship W4250769951A5077979393 @default.
• W4250769951 hasConcept C113196181 @default.
• W4250769951 hasConcept C12554922 @default.
• W4250769951 hasConcept C13965031 @default.
• W4250769951 hasConcept C178790620 @default.
• W4250769951 hasConcept C181911157 @default.
• W4250769951 hasConcept C185592680 @default.
• W4250769951 hasConcept C188053792 @default.
• W4250769951 hasConcept C2778385715 @default.
• W4250769951 hasConcept C2778695967 @default.
• W4250769951 hasConcept C3018436504 @default.
• W4250769951 hasConcept C41625074 @default.
• W4250769951 hasConcept C43617362 @default.
• W4250769951 hasConcept C519581460 @default.
• W4250769951 hasConcept C537181965 @default.
• W4250769951 hasConcept C55493867 @default.
• W4250769951 hasConcept C86803240 @default.
• W4250769951 hasConcept C99975950 @default.
• W4250769951 hasConceptScore W4250769951C113196181 @default.
• W4250769951 hasConceptScore W4250769951C12554922 @default.
• W4250769951 hasConceptScore W4250769951C13965031 @default.
• W4250769951 hasConceptScore W4250769951C178790620 @default.
• W4250769951 hasConceptScore W4250769951C181911157 @default.
• W4250769951 hasConceptScore W4250769951C185592680 @default.
• W4250769951 hasConceptScore W4250769951C188053792 @default.
• W4250769951 hasConceptScore W4250769951C2778385715 @default.
• W4250769951 hasConceptScore W4250769951C2778695967 @default.
• W4250769951 hasConceptScore W4250769951C3018436504 @default.
• W4250769951 hasConceptScore W4250769951C41625074 @default.
• W4250769951 hasConceptScore W4250769951C43617362 @default.
• W4250769951 hasConceptScore W4250769951C519581460 @default.
• W4250769951 hasConceptScore W4250769951C537181965 @default.
• W4250769951 hasConceptScore W4250769951C55493867 @default.
• W4250769951 hasConceptScore W4250769951C86803240 @default.
• W4250769951 hasConceptScore W4250769951C99975950 @default.
• W4250769951 hasIssue "5" @default.
• W4250769951 hasLocation W42507699511 @default.
• W4250769951 hasOpenAccess W4250769951 @default.
• W4250769951 hasPrimaryLocation W42507699511 @default.
• W4250769951 hasRelatedWork W15461257 @default.

• next