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- W4250791344 abstract "The in-depth imaging properties of two-photon excitation microscopy were investigated and compared with those of confocal microscopy. Confocal imaging enabled the recording of images from dental biofilm down to a depth of 40 μm, while two-photon excitation images could be recorded at depths greater than 100 μm. Two-photon excitation point spread functions (PSFs) were recorded at depths ranging from 0 to 90 μm depth using 220-nm diameter fluorescent beads immersed in water. PSFs were measured using both a high numerical aperture oil immersion objective and a water immersion objective. The experiments carried out using the oil immersion objective showed a rapid degradation of both the axial and lateral resolution due to spherical aberrations. In addition, the detected fluorescence intensity rapidly decreased as a function of depth. The experiments carried out using the water immersion objective showed no significant degradation of both the axial and lateral resolution and the fluorescence intensity.Microsc. Res. Tech. 47:206–209, 1999. © 1999 Wiley-Liss, Inc." @default.
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- W4250791344 date "1999-11-01" @default.
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- W4250791344 title "Imaging of optically thick specimen using two‐photon excitation microscopy" @default.
- W4250791344 doi "https://doi.org/10.1002/(sici)1097-0029(19991101)47:3<206::aid-jemt6>3.3.co;2-8" @default.
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