FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4250811985> ?p ?o ?g. }

• W4250811985 abstract "Abstract Background: Rapid diagnostic tests (RDT) are commonly used for the diagnosis of Plasmodium falciparum malaria. However, false negative results of RDT caused by genetic variations of P. falciparum histidine-rich protein 2 and 3 genes ( pfhrp2/3) threaten existing malaria case management and control efforts. The main objective of this study was to investigate the genetic variations of the pfhrp2/3 genes. Methods: A cross-sectional study was conducted from malaria symptomatic individuals in 2018 in Assosa zone, Ethiopia. Finger prick samples were collected for RDT and microscopic examination of thick and thin blood films. Dried blood spots (DBS) were used for genomic parasite DNA extraction and molecular detection. Amplification of parasite DNA was made by quantitative PCR. DNA amplicons of pfhrp2/3 were purified and sequenced. Results: The PfHRP2 repeat type isolates were less conserved compared to the PfHRP3 repeat type. A total of eleven and eight different PfHRP2 and PfHRP3 amino acid repeat types were identified, respectively. Type 1, 4 and 7 repeats were shared by PfHRP2 and PfHRP3 isolates. Type 2 repeats were found only in PfHRP2, while types 16 and 17 were found only in PfHRP3 with a high frequency in all isolates. 18 novel repeat types were found in PfHRP2 and 13 novel repeat types were found in PfHRP3 in single or multiple copies per isolate. The positivity rate for PfHRP2 RDT was high, 82.9% in PfHRP2 and 84.3 % in PfHRP3 sequence isolate at parasitemia levels >250 parasites/µl. Using the Baker model, 100 % of the isolates in group A and 73.7% of the isolates in group B were predicted to be detected by PfHRP2 RDT at parasitemia level> 250 parasite/μl. Conclusion: The findings of this study indicate the presence of different PfHRP2 and PfHRP3 amino acid repeat including novel repeats in P. falciparum from Ethiopia. These results indicate that there is a need to closely monitor the performance of PfHRP2 RDT associated with the genetic variation of the pfhrp2 and pfhrp3 gene in P. falciparum isolates at the country-wide level." @default.
• W4250811985 created "2022-05-12" @default.
• W4250811985 creator A5001583217 @default.
• W4250811985 creator A5022518932 @default.
• W4250811985 creator A5053392395 @default.
• W4250811985 creator A5061075435 @default.
• W4250811985 creator A5069209984 @default.
• W4250811985 creator A5075377318 @default.
• W4250811985 date "2021-06-09" @default.
• W4250811985 modified "2023-10-16" @default.
• W4250811985 title "Genetic Variations of Plasmodium Falciparum Histidine-rich Protein 2 and 3 in Assosa Zone, Ethiopia: Its Impact on the Performance of Malaria Rapid Diagnostic Tests" @default.
• W4250811985 doi "https://doi.org/10.21203/rs.3.rs-587442/v1" @default.
• W4250811985 hasPublicationYear "2021" @default.
• W4250811985 type Work @default.
• W4250811985 citedByCount "0" @default.
• W4250811985 crossrefType "posted-content" @default.
• W4250811985 hasAuthorship W4250811985A5001583217 @default.
• W4250811985 hasAuthorship W4250811985A5022518932 @default.
• W4250811985 hasAuthorship W4250811985A5053392395 @default.
• W4250811985 hasAuthorship W4250811985A5061075435 @default.
• W4250811985 hasAuthorship W4250811985A5069209984 @default.
• W4250811985 hasAuthorship W4250811985A5075377318 @default.
• W4250811985 hasBestOaLocation W42508119851 @default.
• W4250811985 hasConcept C104317684 @default.
• W4250811985 hasConcept C136764020 @default.
• W4250811985 hasConcept C157431215 @default.
• W4250811985 hasConcept C159047783 @default.
• W4250811985 hasConcept C17757408 @default.
• W4250811985 hasConcept C203014093 @default.
• W4250811985 hasConcept C2775867548 @default.
• W4250811985 hasConcept C2778048844 @default.
• W4250811985 hasConcept C2778371730 @default.
• W4250811985 hasConcept C41008148 @default.
• W4250811985 hasConcept C49105822 @default.
• W4250811985 hasConcept C54355233 @default.
• W4250811985 hasConcept C71928629 @default.
• W4250811985 hasConcept C78063203 @default.
• W4250811985 hasConcept C8185291 @default.
• W4250811985 hasConcept C86803240 @default.
• W4250811985 hasConceptScore W4250811985C104317684 @default.
• W4250811985 hasConceptScore W4250811985C136764020 @default.
• W4250811985 hasConceptScore W4250811985C157431215 @default.
• W4250811985 hasConceptScore W4250811985C159047783 @default.
• W4250811985 hasConceptScore W4250811985C17757408 @default.
• W4250811985 hasConceptScore W4250811985C203014093 @default.
• W4250811985 hasConceptScore W4250811985C2775867548 @default.
• W4250811985 hasConceptScore W4250811985C2778048844 @default.
• W4250811985 hasConceptScore W4250811985C2778371730 @default.
• W4250811985 hasConceptScore W4250811985C41008148 @default.
• W4250811985 hasConceptScore W4250811985C49105822 @default.
• W4250811985 hasConceptScore W4250811985C54355233 @default.
• W4250811985 hasConceptScore W4250811985C71928629 @default.
• W4250811985 hasConceptScore W4250811985C78063203 @default.
• W4250811985 hasConceptScore W4250811985C8185291 @default.
• W4250811985 hasConceptScore W4250811985C86803240 @default.
• W4250811985 hasLocation W42508119851 @default.
• W4250811985 hasOpenAccess W4250811985 @default.
• W4250811985 hasPrimaryLocation W42508119851 @default.
• W4250811985 hasRelatedWork W1964955161 @default.
• W4250811985 hasRelatedWork W2117123380 @default.
• W4250811985 hasRelatedWork W2120456640 @default.
• W4250811985 hasRelatedWork W2121172260 @default.
• W4250811985 hasRelatedWork W2136738350 @default.
• W4250811985 hasRelatedWork W2461548932 @default.
• W4250811985 hasRelatedWork W2620159617 @default.
• W4250811985 hasRelatedWork W2778565363 @default.
• W4250811985 hasRelatedWork W2909978988 @default.
• W4250811985 hasRelatedWork W2188201755 @default.
• W4250811985 isParatext "false" @default.
• W4250811985 isRetracted "false" @default.
• W4250811985 workType "article" @default.