Matches in SemOpenAlex for { <https://semopenalex.org/work/W4250868319> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W4250868319 endingPage "1186" @default.
- W4250868319 startingPage "1177" @default.
- W4250868319 abstract "Estrogens increase the proliferative response of estrogen-responsive breast cancer cells to insulin-like growth factors (IGFs). The mechanisms involved are unclear, but the observation that estradiol increases type I IGF receptor levels in MCF-7 breast cancer cells has suggested that the increased response may be due to increased expression of type I IGF receptor. The purpose of this study was to investigate this hypothesis by using a retroviral expression vector to constitutively over-express the type I IGF receptor in estrogen-responsive breast cancer cells. We isolated clones of infected MCF-7 cells that expressed up to 4.5-fold more receptor than the estradiol-induced level in cells infected with a control vector. Hybridization of a type I IGF receptor complementary DNA probe to RNA extracted from these clones showed that most of the receptor RNA was transcribed from the retroviral provirus. Estrogen receptor continued to be expressed in clones overexpressing type I IGF receptor, and overexpression had little effect on the induction of an estrogen-regulated gene by estradiol and the proliferative response to IGFs alone or estradiol alone. Overexpression did, however, alter the proliferative response to IGFs in the presence of estradiol. The three clones analyzed showed an increased sensitivity to low IGF-I concentrations and a paradoxical attenuation of the synergistic effect between estradiol and IGF-I at high IGF-I concentrations. Collectively, these experiments show that the level of expression of the type I IGF receptor is an important determinant in the responsiveness of breast cancer cells to estrogen, but the observation that the response of cells to estradiol alone is not affected by constitutive overexpression of the type I IGF receptor suggests that estrogens stimulate the proliferation of breast cancer cells by regulating the expression of genes in addition to the type I IGF receptor." @default.
- W4250868319 created "2022-05-12" @default.
- W4250868319 creator A5017249221 @default.
- W4250868319 date "1996-04-01" @default.
- W4250868319 modified "2023-10-18" @default.
- W4250868319 title "Paradoxical effects of overexpression of the type I insulin-like growth factor (IGF) receptor on the responsiveness of human breast cancer cells to IGFs and estradiol" @default.
- W4250868319 doi "https://doi.org/10.1210/en.137.4.1177" @default.
- W4250868319 hasPublicationYear "1996" @default.
- W4250868319 type Work @default.
- W4250868319 citedByCount "5" @default.
- W4250868319 countsByYear W42508683192013 @default.
- W4250868319 crossrefType "journal-article" @default.
- W4250868319 hasAuthorship W4250868319A5017249221 @default.
- W4250868319 hasBestOaLocation W42508683191 @default.
- W4250868319 hasConcept C121608353 @default.
- W4250868319 hasConcept C126322002 @default.
- W4250868319 hasConcept C134018914 @default.
- W4250868319 hasConcept C170493617 @default.
- W4250868319 hasConcept C201729690 @default.
- W4250868319 hasConcept C2775960820 @default.
- W4250868319 hasConcept C2777164284 @default.
- W4250868319 hasConcept C2779069268 @default.
- W4250868319 hasConcept C2780689927 @default.
- W4250868319 hasConcept C2908605954 @default.
- W4250868319 hasConcept C502942594 @default.
- W4250868319 hasConcept C530470458 @default.
- W4250868319 hasConcept C71924100 @default.
- W4250868319 hasConcept C84606932 @default.
- W4250868319 hasConcept C86803240 @default.
- W4250868319 hasConcept C96232424 @default.
- W4250868319 hasConceptScore W4250868319C121608353 @default.
- W4250868319 hasConceptScore W4250868319C126322002 @default.
- W4250868319 hasConceptScore W4250868319C134018914 @default.
- W4250868319 hasConceptScore W4250868319C170493617 @default.
- W4250868319 hasConceptScore W4250868319C201729690 @default.
- W4250868319 hasConceptScore W4250868319C2775960820 @default.
- W4250868319 hasConceptScore W4250868319C2777164284 @default.
- W4250868319 hasConceptScore W4250868319C2779069268 @default.
- W4250868319 hasConceptScore W4250868319C2780689927 @default.
- W4250868319 hasConceptScore W4250868319C2908605954 @default.
- W4250868319 hasConceptScore W4250868319C502942594 @default.
- W4250868319 hasConceptScore W4250868319C530470458 @default.
- W4250868319 hasConceptScore W4250868319C71924100 @default.
- W4250868319 hasConceptScore W4250868319C84606932 @default.
- W4250868319 hasConceptScore W4250868319C86803240 @default.
- W4250868319 hasConceptScore W4250868319C96232424 @default.
- W4250868319 hasIssue "4" @default.
- W4250868319 hasLocation W42508683191 @default.
- W4250868319 hasOpenAccess W4250868319 @default.
- W4250868319 hasPrimaryLocation W42508683191 @default.
- W4250868319 hasRelatedWork W2000727265 @default.
- W4250868319 hasRelatedWork W2046622327 @default.
- W4250868319 hasRelatedWork W2051039150 @default.
- W4250868319 hasRelatedWork W2098742882 @default.
- W4250868319 hasRelatedWork W2126532023 @default.
- W4250868319 hasRelatedWork W2128683464 @default.
- W4250868319 hasRelatedWork W2130332764 @default.
- W4250868319 hasRelatedWork W2334410700 @default.
- W4250868319 hasRelatedWork W4236502113 @default.
- W4250868319 hasRelatedWork W1995193459 @default.
- W4250868319 hasVolume "137" @default.
- W4250868319 isParatext "false" @default.
- W4250868319 isRetracted "false" @default.
- W4250868319 workType "article" @default.