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- W4251008093 abstract "Abstract Background: miRNAs regulate circadian patterns by modulating animal biological clock. Clock genes exhibited a cosine expression pattern in the fallopian tube of chicken uterus in our previous study. Clock-controlled miRNAs are present in mammals and Drosophila ; however, whether there are clock-controlled miRNAs in chicken uterus and, if so, how they regulate egg-laying rhythms are not clear. Here, we selected 18 layer hens with similar ovipositional rhythmicity (three birds were sacrificed for study per at 4 h intervals throughout 24 h); their transcriptomes were scanned to identify the circadian miRNAs and to explore regulatory mechanisms within the uterus of chickens. Results: We identified six circadian miRNAs mainly associated with several biological processes including ion trans-membrane transportation, response to calcium ion, and enrichment of calcium signaling pathways. Verification of experimental results revealed that miR-449c-5p exhibited a cosine expression pattern in chicken uterus. Ca 2+ -transporting ATPase 4 ( ATP2B4 ) in the plasma membrane is the predicted target gene of circadian miR-449c-5p and is highly enriched in the calcium signaling pathway. We speculated that clock-controlled miR-449c-5p regulated Ca 2+ transportation during eggshell calcification in chicken uterus by targeting ATP2B4 . ATP2B4 mRNA and protein were rhythmically expressed in chicken uterus, and dual-luciferase reporter gene assays confirmed that ATP2B4 was directly targeted by miR-449c-5p. miR-449c-5p showed an opposite expression profile with ATP2B4 within a 24h cycle in chicken uterus; it inhibited mRNA and protein expressions of ATP2B4 in uterine tubular gland cells. Additionally, overexpression of ATP2B4 significantly decreased intracellular Ca 2+ concentration ( P < 0.05), while knockdown of ATP2B4 accelerated intracellular Ca 2+ concentrations. Similar results were found after ATP2B4 knockdown by miR-449c-5p. These results indicated that ATP2B4 promoted uterine Ca 2+ trans-epithelial transport. Conclusions: Clock-controlled miR-449c-5p regulates Ca 2+ transport in chicken uterus by targeting ATP2B4 during eggshell calcification." @default.
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- W4251008093 date "2021-02-19" @default.
- W4251008093 modified "2023-10-14" @default.
- W4251008093 title "Circadian miR-449c-5p Regulates Uterine Ca2+ Transport During Eggshell Calcification in Chickens" @default.
- W4251008093 doi "https://doi.org/10.21203/rs.3.rs-213129/v1" @default.
- W4251008093 hasPublicationYear "2021" @default.
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